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Biosensis/MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified/M-1586-100/100 µg
Biosensis/MOAB-2 Mouse Monoclonal antibody to Amyloid beta peptide (A beta 40/42), purified/M-1586-100/100 µg
商品编号：
M-1586-100
品牌：
Biosensis inc
市场价：
¥5940.00
美元价：
3564.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | TheamyloidbetapeptideisderivedfromthecleavageoftheAmyloidprecursorprotein(APP)andvariesinlengthfrom39to43aminoacids.However,theform(s)ofamyloid-betapeptide(Aβ)associatedwiththepathologycharacteristicofAlzheimersdisease(AD)remainsunclear.Inparticular,theneurotoxicityofintraneuronalAβaccumulationisanareaofconsiderableresearchandcontroversyprincipallybecauseantibodiesthoughttobespecificforAβhavebeenshowntoactuallydetectintraneuronalAPPandnotAβexclusively. MOAB-2(mouseIgG2b)isapan-specific,high-titerantibodytoAβresidues1-4asdemonstratedbybiochemicalandimmunohistochemicalanalyses(IHC),andishighlyspecificjusttoamyloidbetapeptide. MOAB-2didnotdetectAPPorAPP-CTFsincellculturemedia/lysates(HEK-APPSweorHEKAPPSwe/BACE1)orinbrainhomogenatesfromtransgenicmiceexpressing5familialAD(FAD)mutation(5xFADmice). UsingIHCon5xFADbraintissue,MOAB-2immunoreactivityco-localizedwithC-terminalantibodiesspecificforAβ40andAβ42.MOAB-2didnotco-localizewitheitherN-orC-terminalantibodiestoAPP.Inaddition,noMOAB-2-immunreactivitywasobservedinthebrainsof5xFAD/BACE-/-mice,althoughsignificantamountsofAPPweredetectedbyN-andC-terminalantibodiestoAPP,aswellasby6E10. Inboth5xFADand3xTgmousebraintissue,MOAB-2co-localizedwithcathepsin-D,aMarkerforacidicorganelles,furtherevidenceforintraneuronalAβ,distinctfromAβassociatedwiththecellmembrane.MOAB-2demonstratedstrongintraneuronalandextra-cellularimmunoreactivityin5xFADand3xTgmousebraintissues. |
| Relatedproducts | BiotinylatedMOAB-2antibody,cat#M-1742-50-B OligomericAβELISAKit,cat#BEK-2215-1P/2P |
| BatchNo. | Seeproductlabel |
| Unitsize | 100µg |
| Antigen | Recombinanthumanamyloidbetaprotein42(Aβ42):DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA |
| AntigenLocation | Epitopemapstoresidues1-4ofhumanamyloidbetapeptide40/42 |
| AntigenLength | 42aminoacids |
| AntibodyType | Monoclonal |
| Isotype | IgG2b,lamBDa |
| Clone | MOAB-2 |
| OtherNames | Beta-APP42;Beta-APP40;Beta-amyloidprotein42;Beta-amyloidprotein40;ABPP;APPI;AmyloidbetaA4protein;MOAB2;MOAB-2;Alzheimer"santibody;AB40;AB42;abeta |
| Accession | P05067A4_HUMAN; |
| Producedin | Mouse |
| MolecularWeight | WithFormicacidextractionsandstrandardreducedwesternproceduresbeta-amyloidpeptidemigratesbetween3-6kDa(seefigure1). |
| Purity | ThisproductisaProteinApurifiedmouseIgGbin0.02MPotassiumPhosphate,0.15MSodiumChloride,0.01%sodiumazide,pH7.2. |
| Applications | WesternBlotting(WB),Immunohistochemistry(IH),Immunohistochemistry/paraffinembeddedIH(P),Immunoprecipitation(IP),Immunofluorescence(IF),ELISA.AntibodyhasbeentestedinWBusingpurifiedsyntheticbeta-amyloidpreparationsandfromtransgenicmousebrainformicacidextracts(seefigure1).Formicacidextraction/concentrationisrequiredforwesternblotdetectionfromextracts.MOAB-2antibodyisspecificforbeta-amyloidanddoesnotdetectAPP.Suggesteddilutionof1:2000-1:5,000forWB,standardECLdetectionsystems.Tissuesamplesforthedetectionofbeta-amyloidshouldbepreparedasdetailedinK.L.Youmansetal.{JournalofNeuroscienceMethods196(2011)5159}forbestresults.Detectionofbeta-amyloid40/42indirectwesternscanbedifficult;Dot-blotsofpreparedsamplesarerecommendedasdetailedinYoumans.KLetal2012.IRorfluorescentdetectionsystemsnotyettested,theybutareexpectedtoworkwellwithhigherprimaryantibodydilutionsbecauseoftheincreasedsensitivityofthedetectionmethods.SuggesteddilutionsforIHCare1:50-1:1,000.Freshfrozen,4%paraformaldehydefixedfrozen,orformalinfixedparaffinembeddedtissuesareallsuitable.Optimaldilutionsmustbedeterminedbytheenduser.Antigenretrievalisrequiredinfixedtissuesforoptimalstaining.Antibodywastestedon4%paraformaldehyde/0.1%glutaraldehydefixedfrozentissuefrom3xTgand5xFADmice.MOAB-2antibodydetectsintraneuronalandextracellularbeta-amyloidinIHCanddoesnotdetectAPP{YoumansKLetal2012}.Theantibodyalsoreactswitharchivalformalin-fixed,paraffin-embeddedtissuesampleswithantigenHeatInducedEpitopeRetrieval(HIER):RecommendedCitrate,pH6.0bufferforHIER.Signalwasweakwithoutantigenretrieval.Immunoreactivelywasexpressedinintraneural-amyloiddeposition(plaque)inAlzheimersbrain.MoAB-2wasfoundtobeextremelycleanandwithanexcellentsignaltonoiseratiowithnoneuro-cellulardiffusivestaining.InadditionMOAB-2demonstratednosignificantdifferencesinAβdetectionusingparaffinfixed,free-floatingsections{YoumansKLetal2012}.Formicacid(FA)treatmentresultedinoptimaldetectionofbothintraneuronalandextracellularAβcomparedtowithoutFA(incubatedin88%FA8min,YoumansKLetal2012).FreefloatingtissuesectionswerepermeABIlizedinTBScontaining0.25%TritonX-100(TBSX;3×10min),blockedwith3%horseseruminTBSX(3×10min)followedby1%horseseruminTBSX(2×10min)andincubatedwithappropriateprimaryantibodiesdilutedinTBSXcontaining1%horseserumovernight.SeeYoumansKLetal2012forfullIH(P)protocolandmethoddetails.ForIF,suggesteddilutionis1:100-1:500.Theantibodywastestedon4%PFAfixedfrozentissue.FixedtissueswerewashedinTBS(3×10min),thenincubatedin88%FA(8min),andthenpermeabilizedinTBSX(3×10min),andblockedinTBSXcontaining5%bovineserumalbumin(BSA;1hr).SectionsweresubsequentlyincubatedwithappropriateprimaryantibodiesdilutedinTBSXcontaining2%BSAovernightonanoscillatoryrotator.Detectionwasviafluorescentlylabelledabsorbedsecondaryantibodies{YoumansKLetal2012}.ForIP,thesuggesteddilutionis1:200to1:1,000forlabeledbeta-amyloidusingProteinA/Gconjugatedbeadsasthecapturevehicle{YoumansKLetal2012}.InanELISA,adilutionof1:50-1:1000issuggested.TheantibodyhasbeentestedinELISAsonsyntheticbeta-amyloidandtissuehomogenatesfrombeta-amyloid-Tgmice.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduserforallapplications.Dilutionsprovidedareonlymeanttoserveasabasicguide. |
| Specificity | MOAB-2detectspreparationsenrichedinU-,O-,F-Aβ42,andU-Aβ40bydot-blot,andisthusapan-specificAβantibody.However,MOAB-2isselectiveforthemoreneurotoxicAβ42comparedtoAβ40.Indeed,MOAB-2demonstratedatitrationagainstantigenconcentration,anddetectsAβ40at2.5pmolbutU-,O-andFAβb42atantigenconcentrationsaslowas~0.1pmol{Youmans.KLetal2012}.MOAB-2doesnotdetectAPP(Amyloidprecursorprotein). |
| SpeciesAgainst | Human |
| AntibodyAgainst | Human |
| Cross-reactivity | Human,Rat,otherspeciesnotyettested.ByDotblot,MOAB-2detectedratAβ40andhumanAβ40,albeitwithlessaffinitythanforAβ42.{Youmans.KLetal2012} |
| BlastURL | Clickhere |
| Form | Lyophilized,fromaProteinApurifiedpreparationin0.02MPotassiumPhosphate,0.15MSodiumChloride,0.01%sodiumazide,pH7.2;contains0.01%sodiumazideasapreservative. |
| Appearance | drypowder |
| Reconstitution | Reconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbufferis0.02MPotassiumPhosphate,0.15MSodiumChloride,0.01%sodiumazide,pH7.2. |
| Storage | Afterreconstitutionkeepaliquotsat-20°to-70Cforahigherstability.At4°Ckeepuptooneweek,insulated,protectedfromlight;usesterilemethodsandPipettes.Highlypurifiedglycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles.Keeptightlyclosedwhennotinuseandprotectedfromlight. |
| ExpiryDate | 12monthsafterpurchase |
| SpecificReferences | Zhu,B.etal.(2017)ER-associateddegradationregulatesAlzheimer"samyloidpathologyandmemoryfunctionbymodulatingγ-secretaseactivity.NatCommun.8(1):1472.Application:IHC Huang,TY.etal.(2017)SORLAattenuatesEphA4signalingandamyloidβinducedneurodegeneration.JExpMed.pii:jem.20171413.[Epubaheadofprint].Application:IHC Felecia,M.etal.(2017)PeripheralInflammation,ApolipoproteinE4,andAmyloid-βInteracttoInduceCognitiveandCerebrovascularDysfunction.ASNNeuro.9(4):1759091417719201.Application:IHC/IF Thomas,R.etal.(2016)EpidermalgrowthfactorpreventsAPOE4andamyloid-beta-inducedcognitiveandcerebrovasculardeficitsinfemalemice.ActaNeuropatholCommun.4(1):111Application:IHC Koster,KP.etal.(2016)Epidermalgrowthfactorpreventsoligomericamyloid-βinducedangiogenesisdeficitsinvitro.JCerebBloodFlowMetab.[Epubaheadofprint]Application:IF Löffler,T.etal.(2016)DecreasedPlasmaAβinHyperlipidemicAPPSLTransgenicMiceIsAssociatedwithBBBDysfunction.Front.Neurosci.Application:IF Kobro-Flatmoen,A.etal.(2016)Reelin-immunoreactiveneuronsinentorhinalcortexlayerIIselectivelyexpressintracellularamyloidinearlyAlzheimer"sdisease.NeuroBIOLOGyofDisease.93:172-183.Application:IHC Tai,LM.etal.(2016)TheroleofAPOEincerebrovasculardysfunction.ActaNeuropathol.131(5):709-23.Application:IF Kim,YH.etal.(2015)A3DhumanneuralcellculturesystemformodelingAlzheimer"sdisease.NatProt.10(7):985-1006.Application:WB Condello,C.etal.(2015)MicrogliaconstituteabarrierthatpreventsneurotoxicprotofibrillarAβ42hotspotsaroundplaques.NatCommun.6:6176.Application:IF IulitaMFetal(2014)IntracellularAbetapathologyandearlycognitiveimpairmentsinatransgenicratmodeloverexpressinghumanamyloidprecursorprotein:amultidimensionalstudy.ActaNeuropatholCommun.6:61.Application:IF,IH SmithBRetal(2014)Neuronalinclusionsofalpha-synucleincontributetothepathogenesisofKrabbedisease.JPathol.Apr;235(5):509-21.Application:IF |
| GeneralReferences | TaiLMetal(2016)"TheroleofAPOEincerebrovasculardysfunction."ActaNeuropathol.2016Feb16.[Epubaheadofprint] K.L.Youmansetal(2012)IntraneuronalAbetadetectionin5xFADmicebyanewAbeta-specificantibodyMolNeurodegener.2012Mar16;7(1):8. TaiLMetal(2013)LevelsofsolubleapolipoproteinE/amyloid-β(Aβ)complexarereducedandoligomericAβincreasedwithAPOE4andAlzheimerdiseaseinatransgenicmousemodelandhumansamples.JBiolChem.2013Feb22;288(8):5914-26. K.L.Youmansetal(2011)Amyloid-β42altersapolipoproteinEsolubilityinbrainsofmicewithfivefamilialADmutationsJNeurosciMethods.2011Mar15;196(1):51-9. |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
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