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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum/R-169-100/100 µl
商品详细Biosensis/Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum/R-169-100/100 µl
Biosensis/Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum/R-169-100/100 µl
Biosensis/Rabbit antibody to Peroxiredoxin-6 (NSGPx): whole serum/R-169-100/100 µl
商品编号: R-169-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionFUNCTION:Involvedinredoxregulationofthecell.CanreduceH(2)O(2)andshortchainorganic,fattyacid,andphospholipidhydroperoxides.Mayplayaroleintheregulationofphospholipidturnoveraswellasinprotectionagainstoxidativeinjury. SUBUNIT:Homotetramer.SUBCELLULARLOCATION:Cytoplasm.Lysosome.Alsofoundinlungsecretoryorganelles.MISCELLANEOUS:Theactivesiteistheredox-activeCys-47oxidizedtoCys-SOH.Cys-SOHmayrapidlyreactwithaCys-SHoftheothersubunittoformanintermoleculardisulfidewithaconcomitanthomodimerformation.Theenzymemaybesubsequentlyregeneratedbyreductionofthedisulfidebythioredoxin.MISCELLANEOUS:IrreversIBLyinactivatedbyoveroxidationofCys-47(toCys-SO(3)H)uponoxidativestress.SIMILARITY:BelongstotheahpC/TSAfamily.Rehydrinsubfamily.
BatchNo.Seeproductlabel
Unitsize100µl
AntigenRatrecombinantPeroxiredoxin-6.Thesequenceishomologousinhumanandmouseperoxiredoxin-6.
OtherNamesNon-seleniumglutathioneperoxidase;antioxidantprotein2;1-Cysperoxiredoxin;1-CysPRX;Acidiccalcium-independentphospholipaseA2;NSGPx;Thiol-specificantioxidantprotein;Prdx6;Aipla2;Aop2;Tsa
AccessionPRDX6_RAT
PRDX6_HUMAN
PRDX6_MOUSE
ProducedinRabbit
PurityWholeserum
ApplicationsIHC,WB,ELISA.ThisantibodyworkssuperblyinImmunohistochemistryonfrozenorparaffinembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilutionsforroutineimmunohistochemistryare1:100to1:1000dependingontissueanddetectionmethod.Forwesternblottingadilutionrangeof1:1000to1:4000isrecommended.Adilutionof1:1000to1:4000isrecommendedforELISA.Thisantiserumstainsthecytoplasmofepithelialcellsintheratandmouselungandratandhumanbrainastrocytes.ItstainshumanbrainastrocytesinParkinson'sandAlzheimer'sdiseaseandthecentralcoreofsomeLewybodiesinParkinson'sdiseaseanddementiawithLewybodies.Othertissueshavenotyetbeentested.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThisantibodyhasbeenshowntobespecificforPeroxiredoxin-6protein.
Cross-reactivityRat,humanandmouse,otherspecieshavenotyetbeentested.
BlastitIfyouwouldliketousethisproductinanotherspeicesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis.
FormLyophilised
ReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability.
ExpiryDate12monthsafterpurchase
SpecificReferences1.WalshB.etal.(2010)OverexpressionofPrdx6andresistancetoperoxide-induceddeathinHepa1-6cells:Prdxsuppressionincreasesapoptosis.RedoxRep.2009;14(6):275-84.
2.GardinerF.etal.(2010)InductionofPrdx1andPrdx6inLiverCellsbySerumandTPA.IntlJ.Biol.Vol.2,No.1
References1.KimT.-S,etal.J.Biol.Chem.272:2542-2550(1997).
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3.RushJ,etal.Nat.Biotechnol.23:94-101(2005).
4.WagnerE,etal.Biochem.J.366:777-785(2002).
5.ChenJ.-W,etal.J.Biol.Chem.275:28421-28427(2000).
6.KangS.W,etal.J.Biol.Chem.273:6303-6311(1998).
7.GevaertK,etal.Nat.Biotechnol.21:566-569(2003).
8.GregoryS.G,etal.ature441:315-321(2006).
9.NagaseT,etal.DNARes.2:37-43(1995).
10.FrankS,etal.Oncogene14:915-921(1997).
11.PowerJ,etal.AmJ.Path.161:885-894(2202).
12.PowerJ,etal.ExperimentalLungRes.25:379-391(1999).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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