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当前位置: 首页 > 产品中心 > Functional_antibody > 生物传感器/绵羊谷胱甘肽过氧化物酶1抗体(GPx-1;175-194):全血清/S-072-100/100µl
商品详细生物传感器/绵羊谷胱甘肽过氧化物酶1抗体(GPx-1;175-194):全血清/S-072-100/100µl
生物传感器/绵羊谷胱甘肽过氧化物酶1抗体(GPx-1;175-194):全血清/S-072-100/100µl
生物传感器/绵羊谷胱甘肽过氧化物酶1抗体(GPx-1;175-194):全血清/S-072-100/100µl
商品编号: S-072-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionGlutathioneperoxidase1hasaroleindetoxificationofhydrogenperoxideandisoneofthemostimportantantioxidantenzymesinhumans.Itexistsasahomotetramerwhichlocalisestothecytoplasm.Itbelongstotheglutathioneperoxidasefamily.Glutathioneperoxidase1isoneoffewproteinsinhighervertebratestocontainselenocysteine,whichoccursattheactivesiteofglutathioneperoxidaseandiscodedbyUGA,thatnormallyfunctionsasatranslationterminationcodon.ThisproteinhasapolyalaninesequencepolymorphismintheN-terminalregion,whichincludesthreealleleswithfive,sixorsevenalaninerepeats.Theallelewithfivealaninerepeatsissignificantlyassociatedwithbreastcancerrisk.Twoalternativelysplicedisoformshavebeenidentified.
BatchNo.Seeproductlabel
Unitsize100µl
AntigenAsyntheticpeptide(RRYSRRFQTIDIEPDIEALL)correspondingtotheaminoacids175-194ofhumanlutathioneperoxidase1(GPx-1)conjugatedtodiphtheriatoxinhasbeenusedastheimmunogen.
OtherNamesGSHPx-1;GPx-1;Cellularglutathioneperoxidase;GPX1
AccessionGPX1_HUMAN
ProducedinSheep
PurityWholeserum
ApplicationsIHC,WB.ThisantibodyworkssuperblyinImmunohistochemistryonfrozenorparaffinembeddedtissues.Antigenretrievalhasbeenusedintestingbutmaynotbenecessary.Typicalworkingdilutionsforroutineimmunohistochemistryare1:2000to1:20000dependingontissueanddetectionmethod.Forwesternblottingadilutionrangeof1:10000to1:100000isrecommended.ForELISA,adilutionof1:2000to1:20000isrecommended.Thisantiserumhasextremelyhightitre,itstainshumanandratbrainmicrogliaintenselyandtosomeextentalsostainsneurons.Othertissueshavenotyetbeentested.OnWesternblottingunderreducingconditionsrecognisesa22kDproteininhumanbraintissueandSigmaglutathioneperoxidase(G-4013)isolatedfromhumanerythrocytes.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThisantibodyhasbeenshowntobespecificforglutathioneperoxidase1(GPx-1)protein.
Cross-reactivityHumanandrat,otherspecieshavenotyetbeentested.
BlastitIfyouwouldliketousethisproductinanotherspeicesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis.
FormLyophilised
ReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.Glycerol(1:1)maybeaddedforanadditionalstability.
ExpiryDate12monthsafterpurchase
References1.SukenagaY,etal.NucleicAcidsRes.15:7178-7178(1987).
2.IshidaK,etal.NucleicAcidsRes.15:10051-10051(1987).
3.MullenbachG.T,etal.NucleicAcidsRes.15:5484-5484(1987).
4.ChadaS,etal.Genomics6:268-271(1990).
5.MoscowJ.A,etal.J.Biol.Chem.267:5949-5958(1992).
6.ForsbergL,etal.Hum.Mutat.13:294-300(1999).
7.Kote-JaraiZ,etal.ProstateCancerProstaticDis.5:189-192(2002).
8.HamanishiT,etal.Diabetes53:2455-2460(2004).
9.IchimuraY,etal.J.Urol.172:728-732(2004).
10.StrausbergR.L,etal.Proc.Natl.Acad.Sci.U.S.A.99:16899-16903(2002).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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