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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/兔抗MAP1LC3A抗体:全血清/R-146-100/100µl
商品详细Biosensis/兔抗MAP1LC3A抗体:全血清/R-146-100/100µl
Biosensis/兔抗MAP1LC3A抗体:全血清/R-146-100/100µl
Biosensis/兔抗MAP1LC3A抗体:全血清/R-146-100/100µl
商品编号: R-146-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionMAP1AandMAP1Baremicrotubule-associatedproteinwhichmediatethephysicalinteractionsbetweenmicrotubulesandcomponentsoftheCytoskeleton(probablyinvolvedinautophagosomeformation).MAP1AandMAP1Beachconsistofaheavychainsubunitand3differentlightchainsubunits(LC1,LC2andLC3).MAP1LC3A isoneofthelightchainsubunitsandcanassociatewitheitherMAP1AorMAP1B.TheprecursorformofMAP1LC3AiscleavedbyAPG4/ATG4BtoformthecytosolicformLC3-1.ThisisactivatedbyAPG7L/ATG7,transferredtoATG3andconjugatedtophospholipidtoformthemembrane-boundform,LC3-II.MAP1LC3Aismostabundantinheart,brain,liver,skeletalmuscleandtestisbutisabsentinthymusandperipheralleukocytes.
BatchNo.Seeproductlabel
Unitsize100µl
AntigenAsyntheticpeptide(RSFADRCKEVQQI)correspondingtotheN-terminalofhumanMAP1LC3AproteinconjugatedtoBlueCarrierProteinhasbeenusedastheimmunogen.ThesequenceishomologouswithmouseandratMAP1LC3A.
OtherNamesMicrotubule-associatedproteins1A/1Blightchain3A;MAP1A/MAP1BLC3A;MAP1A/1Blightchain3A;MAP1lightchain3-likeprotein1;Microtubule-associatedprotein1lightchain3alpha;Autophagy-relatedproteinLC3A;Autophagy-relatedubiquitin-likemodifierLC3A;APG8a;MAP1LC3A
AccessionMLP3A_HUMAN
MLP3A_MOUSE
MLP3A_RAT
ProducedinRabbit
MolecularWeight~20kDauncleaved,14-18kDacleaved,SDS-PAGE
PurityWholeserum
ApplicationsIHC,immunohistochemistryon4%PFAfixedor2%PLPfixation,1:100-1000primaryantibodydilution.IC,Immunocytochemistry:acetonefixedspecimensrecommended.1:100-1:1000primaryantibodydilution.WesternBlot:R-146-100requiressamplestobedenaturedONLYbyboilinginSDSsolution,notreduced.Nosignalisachievedusingreducedsamples.(SamplestreatedwithDTTorBetamercaptoethanol).1:500overnight4degreesisrecommendedforprimaryantibodydilutioninwesternblots.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityIHC,WBandELISAconfirmedthespecificityforMAP1LC3A.
Cross-reactivityHuman,rat.Otherspeciesnotyettested.
FormLyophilised
ReconstitutionReconstitutein100µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionkeepaliquotsat-20°CforhigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles.
ExpiryDate12monthsafterpurchase
References1.TheMGCProjectTeam.GenomeRes.14:2121-2127(2004)
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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