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当前位置: 首页 > 产品中心 > Functional_antibody > 针对巢蛋白[4D11](317-630)/M-1385-100/100µl的Biosensis/小鼠单克隆抗体
商品详细针对巢蛋白[4D11](317-630)/M-1385-100/100µl的Biosensis/小鼠单克隆抗体
针对巢蛋白[4D11](317-630)/M-1385-100/100µl的Biosensis/小鼠单克隆抗体
针对巢蛋白[4D11](317-630)/M-1385-100/100µl的Biosensis/小鼠单克隆抗体
商品编号: M-1385-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionNestinisamemberoftheclassIVintermediatefilamentproteinfamilywhichisexpressedinneuronalstemcells.ThemolecularweightofhumanNestinasdeterminedbySDS-PAGEmobilityisabout240kDa.Howevertherealmolecularweightisconsiderablylessthanthis,at177kDa,thedisparitybeinglikelyduetothehighlychargedregionoftheC-terminalsegment.Nestinisrelativelypoorlyconservedinproteinsequenceacrossspeciesboundaries,sothatthemouseandhumanproteinshaveanoverallidentityofonly62%.Asaresultantibodiestothehumanproteinoftenfailtorecognizetherodenthomologueandviceversa.HoweverthisantibodystainsbothrodentandhumanNestin.AntibodiestoNestinarewidelyusedtoidentifyneuralstemcells.
BatchNo.Seeproductlabel
Unitsize100µl
AntigenPartialsegment(region317-630aa)ofhumanNestinexpressedinE.coli
AntigenLocation317-630
AntibodyTypeMonoclonal
IsotypeIgG
Clone4D11
OtherNamesNestin;NES;
AccessionP48681NEST_HUMAN;
ProducedinMouse,IgG1kappa
PurityAscitesfluid
ApplicationsWesternBlotting(WB),Immunocytochemistry(IC)andFlowCytometry.SuggesteddilutionforWBis1:1,000-5,000and1:250-500forIC.Use2ug/10^6cellsforFlowCytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
CommentsReady-to-usereagentforin-vitrolaboratoryresearchuseonly.
SpecificityThisantibodyisspecificforthe240kDaNestinproteinbyWBondevelopingratbrain(P18)homogenate.Amuchweakerbandatapprox.90kDamayalsobeseen.Thisissuggestedtobeabreakdownproductofthe240kDaband.
AntibodyAgainstNestin
Cross-reactivityHuman,Rodent
BlastURLClickhere
FormLyophilisedfromascitesfluid.
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
SpecificReferencesSchomannT,MezzanotteL,DeGrootJCMJ,RivoltaMN,HendriksSH,FrijnsJHM,HuismanMA(2017)Neuronaldifferentiationofhair-follicle-bulge-derivedstemcellsco-culturedwithmousecochlearmodiolusexplants.PLosOne.12(10):e0187183.Application:ICC/IF;Species:Mouse,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs).

GhoCG,SchomannT,deGrootSC,FrijnsJH,RivoltaMN,NeumannMH,HuismanMA.(2015)Isolation,expansionandneuraldifferentiationofstemcellsfromhumanpluckedhair-afurthersteptowardsautologousnerverecovery.CytotechnologyInpress.Application:IF;Species:Human,Hairfolliclebulge-derivedneuralcrest-derivedstemcells(HFBSCs),Keywords:Hairfolliclestemcell,Regeneration,Neuralcrest,Neuron,Glia,Cryopreservation
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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