Biosensis/兔抗缺氧诱导因子-1α（775-790）多克隆抗体：亲和纯化/R-1061-100/100µg-免疫在线蚂蚁淘旗下平台

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Description	Thisproductisnolongeravailable.Pleaseseebelowforourrecommendedalternativeproducts.
Replacedby	R-1722-100
BatchNo.	Seeproductlabel
Unitsize	100µg
Antigen	Asyntheticpeptide(PSDLACRLLGQSMDES)correspondingtoaregion(775-790)fromhumanHypoxia-inducIBLefactor1alpha.Toenhancetheimmunologicalresponse,thispeptidewascoupledtocarrierproteinBSA.
OtherNames	HIF-1alpha;HIF1alpha;ARNT-interactingprotein;MemberofPASprotein1;Basic-helix-loop-helix-PASproteinMOP1;HIF1A;MOP1;
Accession	Q16665HIF1A_HUMAN;
Producedin	Rabbit
Purity	Affinitypurifiedonantigencolumn
Applications	WesternBlotting(WB).Aconcentrationof2.0µg/mlisrecommendedforWB.HumanHIF-1alpha(isoform1)hasapredictedlengthof826residuesandaMWof93kDa.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
Specificity	ThespecificityofthisantibodyhasbeenconfirmedbyWBagainsttheantigen.
Cross-reactivity	Human;mouse;rat;
Blastit	Ifyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechoosecarefully,asthesequenceyouchoosemaybeaprecursorratherthanthematureproteinforexample.
Form	Lyophilisedwith5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3
Reconstitution	Reconstitutein100µlofsteriledistilledwatertoachieveanantibodyconcentrationof1mg/ml.Centrifugetoremoveanyinsolublematerial.
Storage	Atleast12monthsafterpurchaseat2-4°C(lyophilizedformulations).Afterreconstitution,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles
ExpiryDate	12monthsafterpurchase