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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/鼠肌动蛋白单克隆抗体:IgG/M-1646-100/1 Ea
商品详细Biosensis/鼠肌动蛋白单克隆抗体:IgG/M-1646-100/1 Ea
Biosensis/鼠肌动蛋白单克隆抗体:IgG/M-1646-100/1 Ea
Biosensis/鼠肌动蛋白单克隆抗体:IgG/M-1646-100/1 Ea
商品编号: M-1646-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Description Actinisoneofthemostabundantandhighlyconservedproteinsofeukaryotes.Mammalianactinsaretheproductofsixdifferentgeneswithdifferingdistributionpatternsincelltypesandintissues.Themolecularweightofallsixproteinsis42kDa,andoneormoreactinsisfoundinessentiallyeverytypeofcrudecellularandtissueextract.Asaresultantibodiestoactinarewidelyusedasinwesternblottingstandards.Thesecanbeusedtoverifythatthevariousstepsofthewesternblottingprocedurehavebeenperformedcorrectly.Inaddition,actinisregardedasa"housekeeping"proteinwhichisgenerallynotalteredmuchinexpressionasaresultofexperimentalmanipulations.Soquantitationoftheactinbandonthewesternisusedasastandardagainstwiththebanddensityofotherproteinscanbecompared.Themonoclonalbindsallsixactinisotypes(ACTA1,ACTA2,ACTC1,ACTB,ACTG1andACTG2)verystronglyonwesternblots.Itisaveryeffectiveblottingstandardwhichcanworkonanycelltypeortissueextract.Italsoworksinimmunocytochemicalexperiments,bindingstronglyandcleanlytofilopodia,membranerufflesandstressfibers,allknowntoberichinactin.
AntigenActinpreparedfrombovinebrain.
AntibodyTypeMonoclonal
IsotypeIgG1
ProducedinMouse
ApplicationsWesternBlotting(WB),Immunocytochemistry(IC)andFlowcytometry.Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Use2ug/10^6cellsforFlowcytometry.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityTheantibodyreactswitha42kDabandbyWesternblotonacrudeextractfromHeLacells.Ithasalsobeenusedsuccessfullyforimmunocytochemistry.
SpeciesAgainstHuman,rat,mouse,porcineandbovine.Itisexpectedthatitwillworkonothermammaltissues.
AntibodyAgainstActin
FormLyophilizedfromPBS.Contains5%trehalose.
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
GeneralReferences1.Vandekerckhove,J.andWeber,K.Atleastsixdifferentactinsareexpressedinahighermammal:ananalysisbasedontheaminoacid.sequenceoftheamino-terminaltrypticpeptide.J.Mol.Biol.126:783–802(1978).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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