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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Mouse monoclonal antibody to Fibrillarin (Nop1p) [38F3]/M-1372-250/250 µL
商品详细Biosensis/Mouse monoclonal antibody to Fibrillarin (Nop1p) [38F3]/M-1372-250/250 µL
Biosensis/Mouse monoclonal antibody to Fibrillarin (Nop1p) [38F3]/M-1372-250/250 µL
Biosensis/Mouse monoclonal antibody to Fibrillarin (Nop1p) [38F3]/M-1372-250/250 µL
商品编号: M-1372-250
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionFibrillarinispartofthesmallsubunitprocessomecomplex,involvedintheprocessingofpre-18SribosomalRNA.Nop1pistheyeasthomologueoffibrillarin.Fibrillarin/Nop1pisextraordinarilyconserved,sothattheyeastandhumanproteinsare67%identical,andthehumanproteincanfunctionallyreplacetheyeastprotein.Thismeansthatsuitablycross-reactiveantibodiestoNop1p/fibrillarinsuchasthisantibodycanbeusedtorevealnucleoliandstudyfibrillarin/Nop1pinalleukaryotesandarcheatestedtodate.ThisantibodyisbecomingwidelyusedasaconvenientMarkerfornucleoliinawidevarietyofspecies.
BatchNo.Seeproductlabel.
Unitsize250µL
AntigenYeastnuclearpreparations.Hybridomaswerescreenedbyimmunofluorescenceonyeastcellsandbywesternblottingonyeastproteinhomogenates(S.cerevisiae).
AntibodyTypeMonoclonal.
Clone38F3
OtherNamesrRNA2"-O-methyltransferasefibrillarin;34kDanucleolarsclerodermaantigen;FBL;FIB1;FLRN;Nop1p
AccessionP15646FBRL_YEAST;P22087FBRL_HUMAN;
ProducedinMouse
PurityConcentratedcellculturesupernatant.
ApplicationsWesternBlotting(WB),Immunocytochemistry(IC).Adilutionof1:2000(celllysates)to1:10000(nuclearfractions)isrecommendedforWBofyeastproteinsamplesfollowedbychemiluminescentdetection(ECL).Forothernon-ECLWBmethods,adilutionof1:1000to1:5000isrecommended.Adilutionof1:500isrecommendedforECLWBonmammalianfibrillarin.Adilutionof1:1000to1:5000isrecommendedforIConyeastcellsand1:500formammaliancells.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWB.Thisclonewasselectedbecauseitisspecificforthe~34kDaFibrillarin.Itstainsasinglebandonwesternblottingandshowsaclearandstrongpunctatestainingofyeastnuclei.Itcanthereforebeusedasamarkerfornucleoliinawidevarietyofspecies.
AntibodyAgainstFibrillarin(Nop1p)
Cross-reactivityHuman,Rat,Drosophila,S.pombe,C.elegansandandS.pombe.Predictedtoreactwithothermammaliantissues.
FormLyophilized,withoutpreservatives.
ReconstitutionReconstitutein250uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
SpecificReferencesPaeschkeK.etal(2005)Telomereend-bindingproteinscontroltheformationofG-quadruplexDNAstructuresinvivo.NatStructMolBiol.2005Oct;12(10):847-54.

PostbergJ.etal(2005)Synthesisofpre-rRNAandmRNAisdirectedtoachromatin-poorcompartmentinthemacronucleusofthespirotrichousciliateStylonychialemnae.ChromosomeRes.2006;14(2):161-75.

DuT.G.etal(2008)NucleartransitoftheRNA-bindingproteinShe2isrequiredfortranslationalcontroloflocalizedASH1mRNA.EMBORep.2008Aug;9(8):781-7.

SrivastavaL.etal(2010)MammalianDEADboxproteinDdx51actsin3"endmaturationof28SrRNAbypromotingthereleaseofU8snoRNA.MolCellBiol.2010Jun;30(12):2947-56.

SasanoY.etal(2008)DistributionofU3smallnucleolarRNAandfibrillarinduringearlyembryogenesisinCaenorhaBDitiselegans.Biochimie.2008Jun;90(6):898-907.

MengL.etal(2007)Nucleolartraffickingofnucleosteminfamilyproteins:commonversusprotein-specificmechanisms.MolCellBiol.2007Dec;27(24):8670-82.
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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