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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/抗rh-BDNF[4C8]的小鼠单克隆抗体:IgG/M-1744-50/50µg。
商品详细Biosensis/抗rh-BDNF[4C8]的小鼠单克隆抗体:IgG/M-1744-50/50µg。
Biosensis/抗rh-BDNF[4C8]的小鼠单克隆抗体:IgG/M-1744-50/50µg。
Biosensis/抗rh-BDNF[4C8]的小鼠单克隆抗体:IgG/M-1744-50/50µg。
商品编号: M-1744-50
品牌: Biosensis inc
市场价: ¥4540.00
美元价: 2724.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionBDNFbelongstotheneurotrophinfamilyandpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.ItisamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"s,andParkinson"sdisease.MicrogliareleaseBDNFthatmaycontributetoneuroinflammationandneuropathicpain.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.POsttranslationmodification:ConvertedintomatureBDNFbyplasmin(PLG).SIMILARITY:BelongstotheNGF-betafamily.
RelatedproductsAvailableas100µg(2x50µg).
BatchNo.Seeproductlabel.
Unitsize50µg.
AntigenRecombinanthumanmatureBDNFexpressedinE.coli
AntibodyTypeMousemonoclonal.
Clone4C8
OtherNamesBrain-derivedneurotrophicfactor;Abrineurin;proBDNF
AccessionBDNF_HUMAN
ProducedinMouse
MolecularWeightMatureBDNFdimerhasamolecularweightof28kDa.InWesternBlotting,matureBDNFmonomermigratesat14kDa.
PurityProteinGpurifiedmouseIgG.
ApplicationsELISA:50-100ng/mL

WesternBlotting(denaturingandreducing):0.2to2µg/mL.M-1744-50antibodydetects14kDamatureBDNFmonomerinhumanserumhumanandacidtreatedhumanandrodentwholetissuehomogenates.InsimpleTris-homogenatehumanbrainlysates,M-1744-50detectsmatureBDNFat14kDaaswellasanuncharacterizedbandat~50kDa.NoproBDNFbandisdetectedinoursamples,althoughM-1744-50willdetectproBDNFinpurifiedformonblots.Additionally,M-1744-50detectsanadditionalband18kDaBDNFisoformhumanserum.Forcelllysates,somecautionisadvisedasinourhandsM-1744-100failstodetectmatureBDNFonSH-SY5YorC6celllysatesbutdoesdetectmatureBDNFinneuronalcellculturelysates.Thereasonforthesedifferenceshasnotbeencharacterized.FordetectionofmatureBDNFincelllysatessuchasSH-SY5Ywerecommendaffinity-purifiedrabbitpolyclonalantibodytorhBDNF(R-1707-100),orrabbitpolyclonalantibodytoBDNFpeptide1-10(R-083-100,wholeserum;R-066-500,IgG).

Immunofluorescence:1to4µg/mL,4%formaldehydefixationandpermeabilization.Stainingcanbeweakuntiloptimized.

M-1744-50isnotrecommendedforFlowCytometryatthistime.

Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityDetectshuman,mouse,rat,guineapigBDNF.ExpectedtodetectBDNFfromotherspeciesduetosequencehomology.
BlastURLClickhere
FormLyophilizedfromasolutioncontainingPBSpH7.4,3%trehalose,with0.1%sodiumazide.
ReconstitutionSpinvialbrieflybeforeopening.Reconstitutewith50µLsterilewatertoobtainaconcentrationof1mg/mL.
StorageStorelyophilizedantibodyat-20°Cto-80°Cprotectedfrommoisture.Afterreconstitutiondivideantibodyintousefulaliquotsandkeepaliquotsat-20°Cto-80°Cforahigherstability.Workingaliquotscanbekeptat2-8°Cforupto1month.Avoidrepetitivefreeze/thawcycles.
ExpiryDate12monthsafterpurchaseifunopened.
ReferencesInstructionsforAcid-ExtractionofBrainTissue

BDNFAntibodiesforWesternBlotting
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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