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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Rabbit antibody to BDNF (129-138): IgG/R-066-500/500 µg
商品详细Biosensis/Rabbit antibody to BDNF (129-138): IgG/R-066-500/500 µg
Biosensis/Rabbit antibody to BDNF (129-138): IgG/R-066-500/500 µg
Biosensis/Rabbit antibody to BDNF (129-138): IgG/R-066-500/500 µg
商品编号: R-066-500
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionBDNFbelongstotheneurotrophinfamilyandregulatesthesurvivalanddifferentiationofneuronsduringdevelopment.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"s,andParkinson"sdisease.MicrogliareleaseBDNFthatmaycontributetoneuroinflammationandneuropathicpain.
FUNCTION:Promotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.MajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.POsttranslationmodification:ConvertedintomatureBDNFbyplasmin(PLG).SIMILARITY:BelongstotheNGF-betafamily.
BatchNo.Seeproductlabel
Unitsize500µg
AntigenAsyntheticpeptide(HSDPARRGEL)asapartofhumanBDNFprotein(aa:129-138)conjugatedtoKLHhasbeenusedastheimmunogen.TheBDNFproteinsequenceishighlyconservedamongstmammalianspecies.
OtherNamesBrain-derivedneurotrophicfactor;Abrineurin;proBDNF;
AccessionBDNF_HUMAN
ProducedinRabbit
PurityProteinGpurifiedIgG
ApplicationsWesternBlotting:Aconcentrationof1-10µg/mlisrecommendedforthisapplication.InWesternBlotting,thisantibodydetectsmultipleBDNFisoforms(14kDamatureBDNF,18kDaisoform,28kDaBDNFdimer/truncatedBDNF,32kDaproBDNFmonomer)dependingonsampleapplication(humanserum,celllysate,tissuehomogenate).

IHC:Antibodyworkswellinimmunohistochemistrywiththeproperfixation,pretreatmentsanddilution.Formalfixed,paraffinembeddedtissueisnotrecommend.RecommendedfixationisZamboni’sfixativeorlight4%PFAfixationonfixed,frozentissue.Recommendeddilutionis1-10µg/mlforimmunohistochemistryat4degreescentigradefor2-48hours.

ELISA:1-10ug/mLcapture/detection.

Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityLessthan0.1%crossreactivitywithmouseNGF,recombinanthumanNT3andNT4/5hasbeenrecordedbydotblotanalysis.
Cross-reactivityThisantiserumisknowntorecogniserat,humanandhumanBDNF,andisexpectedtoreactwithBDNFfromotherspeciesduetoaminoacidsequencehomology.
BlastitIfyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedIDbetweentheimmunogenusedhereindifferentspeciesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis.
FormLyophilisedfromPBS,pH7.4,withoutpreservatives.
ReconstitutionReconstitutein500µlofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionkeepaliquotsat-20°Cforahigherstability,andat4°Cwithanappropriateantibacterialagent.Glycerol(1:)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles.
ExpiryDate12monthsafterpurchase
SpecificReferencesFeronFetal(2008)Neurotrophinexpressionintheadultolfactoryepithelium.BrainRes.1196:13-21Application:IHC;Species:Rat
GeneralReferences1.AAchesonetal(1995)Nat.74:450-3
2.QYanetal(1994)J.Neurosci.14(9):5281-91
3.XFZhouetal(1996)Neurosci.74:945-53
4.XFZhou,etal(1998)Exp.Neurol.149:237-42
5.BMellstrometal(2004)CritRevNeurobiol16,43-9
6.ITapia-Arancibiaetal(2004)FrontNeuroendocrinol25,77-107
7.SPezet,etal(2002)BrainResBrainResRev40,240-9
8.BardeY.A.etal(1989)EMBOJ.1:549
9.ConnerJetal.(1997)J.Neurosci.17:2295
10.JACoulletal(2005)Nature.Dec15;438(7070):1017-21.
11.CGomesetal(2013)JNeuroinflammation.Jan30;10:16.
ReferencesBDNFAntibodiesforWesternBlotting
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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