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当前位置: 首页 > 产品中心 > Functional_antibody > 生物传感器/兔抗rhBDNF抗体:亲和纯化/R-1707-100/100µg
商品详细生物传感器/兔抗rhBDNF抗体:亲和纯化/R-1707-100/100µg
生物传感器/兔抗rhBDNF抗体:亲和纯化/R-1707-100/100µg
生物传感器/兔抗rhBDNF抗体:亲和纯化/R-1707-100/100µg
商品编号: R-1707-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionBDNFbelongstotheneurotrophinfamilyandpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.ItisamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"s,andParkinson"sdisease.MicrogliareleaseBDNFthatmaycontributetoneuroinflammationandneuropathicpain.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.POsttranslationmodification:ConvertedintomatureBDNFbyplasmin(PLG).SIMILARITY:BelongstotheNGF-betafamily.
BatchNo.Seeproductlabel.
Unitsize100µg
AntigenAntibodywasraisedagainstaGST-taggedrhBDNFfusionproteinandexpressedinandpurifiedfromE.coli.
AntibodyTypeRabbitpolyclonal.
OtherNamesBrain-derivedneurotrophicfactor;Abrineurin
AccessionBDNF_HUMAN
ProducedinRabbit
MolecularWeightMatureBDNFdimerhasamolecularweightof28kDa.InWesternBlotting,matureBDNFmonomermigratesat14kDa.
PurityAffinitypurifiedonantigencolumn.
ApplicationsWesternBlotting(denaturingandreducing):0.2to1µg/mL.Antibodydetects14kDamatureBDNFmonomerand32kDaproBDNFmonomerincelllysateandtissuehomonenate.Antibodyhasonlybeentestedoncelllysateandtissuehomogenateofhumanorigin.Acid-treatedsamplesmaygivecleanerblots,andenhancesignalsforBDNF.R-1707-100isnotrecommendedforhumanserumsamples.Forhumanserumanalysis,werecommendmousemonoclonalantibodytorhBDNF(M-1744-50/100),orrabbitpolyclonalantibodytoBDNFpeptide1-10(R-083-100,wholeserum;R-066-500,IgG).

FlowCytometry:~2µgper10^6cells,methanolfixation.Note:R-1707-100cannotbeusedtodistinguishtheflowcytometrysignaloriginatingfrommatureBDNFversusproBDNF.

Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityHuman,ratandmouseBDNF.ExpectedtodetectBDNFfromotherspeciesduetosequencehomology.
Cross-reactivityNocross-reactivitywithotherneurotrophins.
BlastURLClickhere
FormLyophilizedfromasolutioncontainingPBSpH7.4,3%trehalose,with0.05%sodiumazide.
ReconstitutionSpinvialbrieflybeforeopening.Reconstitutevialwith100µLsterilewatertoobtainaconcentrationof1mg/mL.
StorageStorelyophilizedantibodyat-20°Cto-80°Cprotectedfrommoisture.Afterreconstitutiondivideantibodyintousefulaliquotsandkeepaliquotsat-20°Cto-80°Cforahigherstability.Workingaliquotscanbekeptat2-8°Cforupto1month.Avoidrepetitivefreeze/thawcycles.
ExpiryDate12monthsafterpurchaseifunopened.
ReferencesInstructionsforAcid-ExtractionofBrainTissue

BDNFAntibodiesforWesternBlotting
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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