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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Chicken polyclonal antibody to Growth Associated Protein 43 (GAP43, 217-227): IgY/C-1649-100/100 µL
商品详细Biosensis/Chicken polyclonal antibody to Growth Associated Protein 43 (GAP43, 217-227): IgY/C-1649-100/100 µL
Biosensis/Chicken polyclonal antibody to Growth Associated Protein 43 (GAP43, 217-227): IgY/C-1649-100/100 µL
Biosensis/Chicken polyclonal antibody to Growth Associated Protein 43 (GAP43, 217-227): IgY/C-1649-100/100 µL
商品编号: C-1649-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionGAP43isveryabundantproteinwhichisfoundconcentratedinneurons.Onegroupdiscovereditasoneofthreeproteinswhichbecomesunregulatedduringtheregenerationofthetoadopticnerve(1).ThreeGAPs(Growthassociatedproteins)werediscovered,andthenumber43comesfromtheapparentSDS-PAGEmolecularweightoftheonenamedGAP43.TheHGNCnameforthisproteinis,notsurprisingly,GAP43.LaterworkshowedthatGAP43doesnotrunonSDS-PAGEinafashionwhichaccuratelyreflectsitsmolecularweight,andthatGAP43proteinsfromdifferentspeciesmayrunatdifferentapparentmolecularweights.PartlyduetothesefeaturesGAP43wereindependentlydiscoveredbyseveraldifferentgroupsandthereforehasseveralalternatenames,suchasproteinF1,pp46,neuromodulin,neuralphosphoproteinB-50andcalmodulin-bindingproteinP-57.IneachcasethenumberreflectstheapparentSDS-PAGEmolecularweight,andunderlinestheunusualpropertiesofthismolecule.MammalianGAP43proteinscontainsonly226-243aminoacids,andsotherealmolecularweightis23.61-25.14kDa.GAP43hasbeenextensivelystudiedandisknowntobeamajorproteinkinaseCsubstrateandtobindcalmodulinavidly.GAP43isanchoredtotheplasmamembranebypalmitoylationmodifications.
BatchNo.Seeproductlabel.
Unitsize100µL
AntigenC-terminalpeptide217-227ofratandmouseGAP43,whichisKEDPEADQEHA,towhichanNterminalCysteineresiduewasaddedtoallowchemicalcouplingtoKeyholeLimpetHemocyanincarrierprotein.
AntibodyTypePolyclonal.
IsotypeIgY
AccessionP07936NEUM_RAT
P06837NEUM_MOUSE
ProducedinChicken.
PurityAntibodyissuppliedasanaliquotofIgYpreparation.
ApplicationsWesternBlotting(WB)andImmunocytochemistry(ICC).Adilutionof1:1,000-1:5,000isrecommendedforWB.Adilutionof1:500-1,000isrecommendedforICC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityTheantibodyreactswitha43kDabandbyWesternblotonbovinehippocampuslysate.Ithasalsobeenusedsuccessfullyforimmunocytochemistry.
Cross-reactivityHuman,ratandbovine.Itisexpectedthatitwillworkonothermammaltissuesduetoaminoacidsequencesimilarity.
FormLyophilized,containing3%trehalose,withoutpreservatives.
ReconstitutionReconstitutein100uLsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageStorelyophilised,unopenedvialat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°Cto-80°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase(unopenedvial).
GeneralReferencesSkeneJH,WillardM.Changesinaxonallytransportedproteinsduringaxonregenerationintoadretinalganglioncells.J.CellBiol.89:86-95(1981).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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