抗醛缩酶C/M-1693-100/100μg的Biosensis/小鼠单克隆抗体
商品编号:
M-1693-100
品牌:
Biosensis inc
市场价:
¥5940.00
美元价:
3564.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | AldolasesareglycolyticenzymethatcatalyzesthereversIBLealdolcleavageoffructose1,6-bisphosphateandfructose-1-phosphatetodihydroxyacetonephosphateandeitherglyceraldehyde3-phosphateorglyceraldehyde,respectively.ThreealdolaseisozymesarefoundinmammalsspecificallyaldolasesA,B,andC,eachofwhichisencodedbyaseparategene.AldolaseAisgenerallyconsideredtobeamuscleenzyme.Northernanalysisofculturedcellssuggeststhatitispresentinbothneuronsandglia(1).AldolaseBisconsideredtobealiver-specificenzymeanditistranscriptionallyactivatedbysignalsfromhormonesanddietaryfactors(2).Intheadult,aldolaseCisthebrain-specificisozyme,withlowbutdetectableactivityinfetaltissues(1,3-6).AldolaseCshares81%aminoacididentitywithaldolaseAand70%identitywithaldolaseB.Earlierstudiesusingisozyme-specificantibodiesreportitslocationingraymatterastrocytesandcellsofthepiamater(5,8).Insituhybridizationofmousecentralnervoussystemusingisozyme-specificprobesrevealedthataldolaseAandCareexpressedincomplementarycelltypes:aldolaseAmRNAisfoundinneurons;aldolaseCmessageisdetectedinastrocytes,somecellsofthepiamater,andPurkinjecells(9).AldolaseCcaninsomesituationsbeusedasanastrocyteMarker.HoweverPurkinjecellsofthecerebellumcontainhighlevelsoftheenzyme,sotheenzymeisnottotallyastrocytespecific. |
Unitsize | 100ug |
Antigen | N-terminal20aminoacidsofaldolaseCprotein. |
AntibodyType | Monoclonal |
Isotype | IgG1 |
Producedin | Mouse |
Applications | WesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:1,000-1:2,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | Theantibodyreactswitha40kDabandbyWesternblotonacrudebovinecerebellumhomogenate.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
SpeciesAgainst | Theantibodyhasbeendirectlytestedforreactivityinbovine.Itisexpectedthatitwillworkonothermammaltissues. |
AntibodyAgainst | AldolaseC |
Form | Lyophilised.Theantibodyhasbeenpurifiedfromtissueculturesupernatant. |
Appearance | Lyophilised |
Reconstitution | Reconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles. |
ExpiryDate | 12monthsafterpurchase |
GeneralReferences | 1.PopoviciT,Berwald-NetterY,VibertM,KahnA,SkalaH.LocalizationofaldolaseCmRNAinbraincells.FEBSLett.268,189-193(1990).2.WeberA,MarieJ,CottereauD,SimonM,BesmondC,DreyfusJ.&KahnA.DietaryControlofAldolaseBandL-typePyruvateKinamseRNAsinRat.ÂJ.Biol.Chem259,1798-1802(1984).3.MukaiT,YatsukiH,MasukoS,AraiY,JohK&HoriK.Thestructureofthebrain-specificrataldolaseCgeneanditsregionalexpression.Biochem.Biophys.Res.Commun.174,1035-1042(1991).4.RoydsJ,IronsideJ,WarnaarS,TaylorC&TimperleW.MonoclonalantibodytoaldolaseC:aselectivemarkerforPurkinjecellsinthehumancerebellum.Neuropathol.Appl.Neurobiol.13,11-21(1987).5.ThompsonR.,KynochP.WillsonV.CellularlocalizationofaldolaseCsubunitsinhumanbrain.BrainRes.232,489-493(1982).6.SchapiraF,ReuberM,HatzfeldA.Resurgenceoftwofetal-typeofaldolases(AandC)insomefast-growinghepatomas.Biochem.Biophys.Res.Commun.40,321-327(1970).7.AraiY,KajiharaS,MasudaJ,OhishiS,ZenK,OgataJ.MukaiT.Position-independent,high-level,andcorrectregionalexpressionoftherataldolaseCgeneinthecentralnervoussystemoftransgenicmice.Eur.J.Biochem.221,253-260(1994).8.WachsmuthE,ThornerM.&PfleidererG.Thecellulardistributionofaldolaseisozymesinratkidneyandbraindeterminedintissuesectionsbytheimmuno-histochemicalmethod.Histochemistry,45,143-161(1975).9.WaltherEU,DichgansM,MaricichSM,RomitoRR,YangF,DziennisS,ZacksonS,HawkesR,HerrupK.GenomicsequencesofaldolaseC(ZebrinII)directlacZexpressionexclusivelyinnon-neuronalcellsoftransgenicmice.ProcNatlAcadSciUSA.Mar3;95(5):2615-20(1998). |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
TR-100-BG型
S-1754-500型
神经营养因子
糖尿病与肥胖
细胞内贩运
电话:PE-1239-5
神经肽和受体
B-100-PS型
受体和通道分子
2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话:PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
B-200-PS型
PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
PE-1253-100型
TR-100-FJ
BL-005-500型
神经内分泌
M-1728-100型
DNA损伤与修复
PE-001-100型
PE-1608-1型
TR-400-AG公司
PE-1243-25
细胞标记
PE-1608-5型
TR-100-FJT
自噬
PE-1242-2型
其他神经元
神经递质和酶
抗氧化酶
神经元标志物
标记抗体
BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
显示分类
联络我们