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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/抗腺苷酸环化酶III型(ACIII)的兔多克隆抗体:亲和纯化/R-1687-100/100µg
商品详细Biosensis/抗腺苷酸环化酶III型(ACIII)的兔多克隆抗体:亲和纯化/R-1687-100/100µg
Biosensis/抗腺苷酸环化酶III型(ACIII)的兔多克隆抗体:亲和纯化/R-1687-100/100µg
Biosensis/抗腺苷酸环化酶III型(ACIII)的兔多克隆抗体:亲和纯化/R-1687-100/100µg
商品编号: R-1687-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionAdenylatecyclasesareenzymeswhichinteractwithandareactivatedbytheGTPboundalphasubunitsoftrimericG-proteins.ActivatedadenylatecyclasesareresponsIBLefortheproductionoftheimportant"secondmessenger"signallingmoleculecyclic-AMP,whichisgeneratedfromATP.ThetypeIIIadenylatecyclaseenzymeislocalizedinthemembranessurroundingtheciliainneurons,andourantibodyisanexcellentMarkerofneuronalciliainthebrainandincellsintissueculture.AdenylatecyclasetypeIIIisalargecomplexmoleculeof,inthehuman,1145aminoacidswithadeducedmolecularweightof129kDa.Theproteinmaybevariablyglycosylated,sothatonSDS-PAGEandwesternblotsitrunsasadiffusebandofabout160kDaincortexandabout200kDainolfactoryepithelium.Themoleculehasacomplexstructure,with12transmembranedomainsandtwocyclasedomains.EachcyclasedomainisimmediatelyC-terminalto6transmembranesegments,butonlythesecond,C-terminalcyclaseisbelievedtobecatalyticallyactive.
BatchNo.Seeproductlabel.
Unitsize100µg
Antigen20aminoacidpeptideidenticaltotheC-terminusofratACIII(aminoacidsPAAFPNGSSVTLPHQVVDNP)
AntibodyTypePolyclonal
IsotypeIgG
AccessionP21932ADCY3_RAT
ProducedinRabbit
PurityAffinitypurified
ApplicationsWesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityTheantibodystainsabandatabout200kDainolfactoryepitheliumwhichisrichincilia.Fewerciliaarefoundinfrontalcortex,andtheproteinislessheavilyglycosylated,andalessprominentbandisseenatabout160kDa.Ithasalsobeenusedsuccessfullyforimmunocytochemistryonmixedneuron/gliacultures.
AntibodyAgainstAdenylatecyclasetypeIII
Cross-reactivityTheantibodyhasbeendirectlytestedforreactivityinrat.Itisexpectedthatitwillworkonhumanduetohomologywiththeimmunogenandpossiblyothermammaltissues(Human,horse,cow,pig,chicken,mouse).
FormLyophilisedfromPBSpH7.4,containing3%trehalose,withoutpreservatives.
ReconstitutionReconstitutewith100µLsterile-filtered,ultrapurewater,toachieveanantibodyconcentrationof1mg/mL.Centrifugebrieflytoremoveanyinsolublematerial.
StorageStorelyophilised,unopenedvialat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°Cto-80°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase(unopenedvial).
GeneralReferences1.FuchsJL,SchwarkHD.Neuronalprimarycilia:areview.CellBiolInt.28:111-8(2004).
2.LouviAandGroveEA.CiliaintheCNS:thequietorganelleclaimscenterstage.Neuron69:1046-1060(2011).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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