Biosensis/兔抗人血影蛋白多克隆抗体，αII：全血清/R-1694-100/100µL
商品编号：
R-1694-100
品牌：
Biosensis inc
市场价：
¥5940.00
美元价：
3564.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | ThespectrinfamilyofproteinswereoriginallydiscoveredasmajorcomponentsofthesubmembraneousCytoskeletonofosmoticallylysedredbloodcells(1).Thelysedbloodcellscouldbeseenasclearredbloodcellshapedobjectsinthelightmicroscopeandwerereferredtoasredcell"ghosts".Themajorproteinsoftheseghostsprovedtobeactin,ankyrin,band4.1andseveralotherproteins,includingtwomajorbandsrunningatabout240kDaand260kDaonSDS-PAGEgels.Thispairofbandswasnamed"spectrin"sincetheywerediscoveredintheseredbloodcellghosts(1).Laterworkshowedthatsimilarhighmolecularbandswereseeninmembranepreparationsfromothereukaryoticcelltypes.WorkbyLevineandWillarddescribedapairofabout~240-260kDamolecularweightbandswhichweretransportedattheslowestratealongmammalianaxons(2).Theynamedtheseproteins"fodrin"asantibodystudiesshowedthattheywerelocalizedinthesheathundertheaxonalmembrane,butnotinthecoreoftheaxon(2;fodrosisGreekforsheath).Subsequentlyfodrinwasfoundtobeamemberofthespectrinfamilyofproteins,andthespectrinnomenclatureisnownormallyused(3).Spectrinsformtetramersoftwoalphaandtwobetasubunits,withthealphacorrespondingtothelowermolecularweight~240kDabandandthebetacorrespondingtothe~260kDaorinsomecasemuchlargerband.Mostspectrintetramersareabout0.2micronsor200nmlong,andeachalphaandbetasubunithasacelltypespecificexpressionpattern.Thebasicstructureofeachspectrinsubunitisthespectrinrepeat,whichisasequenceofabout110aminoacidswhichdefinesacompactdomaincontainthreecloselypackedalpha-helices.Eachspectrinsubunitcontainsmultiplecopiesofthisrepeat,with20ineachofthealphasubunits.ThebetaI-IVsubunitseachcontain17spectrinrepeats,whilethebetaVsubunit,alsoknownasbeta-heavyspectrin,contains30oftheserepeats.Thevarioussubunitsalsocontainseveralotherkindsoffunctionaldomain,allowingthespectrintetramertointeractwithavarietyofprotein,ionicandlipidtargets.Thealpha-subunitseachcontainonecalmodulinlikecalciumbindingregionandoneSrc-homology3(SH3)domain,anabundantdomaininvolvedinspecificprotein-proteininteractions.ThebetasubunitsallhaveaN-terminalactinbindingdomainandmayalsohaveoneSH3domainandonepleckstrinhomologydomain,amultifunctionaltypeofbindingdomainwhichinbetaIspectrinatleastbindsthemembranelipidPIP2(5).Spectrinsarebelievedtohaveafunctioningivingmechanicalstrengthtotheplasmamembranesincethetetramersassociatewitheachothertoformadensesubmembraneousgeodesicmeshwork(3).Theyalsobindavarietyofothermembraneproteinsandmembranelipids,andtheproteinstheybindtoarethereforethemselveslocalizedinthemembrane.Diseasesmaybeassociatedwithdefectsinoneorotherofthespectrinsubunits(6).Forexample,someformsofhereditaryspherocytosis,thepresenceofsphericalredbloodcellswhicharepronetolysis,canbetracedtomutationsinsomeofthespectrinsubunits(7).Thealpha-IIsubunitiswidelyexpressedintissuesbut,inthenervoussystem,isfoundpredominantlyinneurons.Theantibodycanthereforebeusedtoidentifyneuronsandfragmentsderivedfromneuronalmembranesincellsintissuecultureandinsectionedmaterial. |
| BatchNo. | Seeviallabel |
| Unitsize | 100µL |
| Antigen | TheantibodywasraisedagainstamixoffiverecombinantconstructscontainingtheentireC-terminalregionofhumanalpha-IIspectrin(aminoacids676-2,400). |
| AntibodyType | Polyclonal |
| Isotype | IgG |
| Accession | Q13813SPTAN1_HUMAN |
| Producedin | Rabbit |
| Purity | Wholeserum. |
| Applications | WesternBlotting(WB)andImmunocytochemistry(IC).SuggesteddilutionforWBis1:5,000-10,000and1:500-1,000forIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Theantibodyreactswitha240kDabandbyWesternblotonmousesciaticnerveextract.Minorbandsbelowmaybeseenandthislikelyrepresentsinvivoproteolyticfragmentsofalpha-IIspectrin.Ithasalsobeenusedsuccessfullyforimmunocytochemistry. |
| SpeciesAgainst | Human.Otherspeciesnotyettested. |
| Form | Lyophilised. |
| Reconstitution | Reconstitutewith100µLsterile-filtered,ultrapurewater.Centrifugebrieflytoremoveanyinsolublematerial. |
| Storage | Storelyophilised,unopenedvialat2-8°Corlower.Afterreconstitution,preparealiquotsandstoreat-20°Cto-80°CforahigherstABIlity.Avoidfreeze-thawcycles. |
| ExpiryDate | 12monthsafterpurchase(unopenedvial). |
| GeneralReferences | 1.MarchesiVT&SteersEJr.Selectivesolubilizationofaproteincomponentoftheredcellmembrane.Science159:203-4(1968). 2.LevineJ&WillardM.Fodrin:axonallytransportedpolypeptidesassociatedwiththeinternalperipheryofmanycells.JCellBiol.90:631-42(1981). 3.BennettV&BainesAJ.Spectrinandankyrin-basedpathways:metazoaninventionsforintegratingcellsintotissues.PhysiolRev.81:1353-92(2001). 4.Djinovic-CarugoK,GautelM,YlänneJ&YoungP.Thespectrinrepeat:astructuralplatformforcytoskeletalproteinassemblies.FEBSLett.513:119-23(2002). 5.Wang,DSandShawG.TheassociationoftheC-terminalregionofbetaIsigmaIIspectrintobrainmembranesismediatedbyaPHdomain,doesnotrequiremembraneproteins,andcoincideswithainositol-1,4,5triphosphatebindingsite.BBRC217:608-15(1995). 6.BennettV&HealyJ.Organizingthefluidmembranebilayer:diseaseslinkedtospectrinandankyrin.TrendsMolMed14:28-36(2008).7.EberS&LuxSE.Hereditaryspherocytosis--defectsinproteinsthatconnectthemembraneskeletontothelipidbilayer.SeminHematol41:118-41(2004). |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
TR-100-BG型
S-1754-500型
神经营养因子
糖尿病与肥胖
细胞内贩运
电话：PE-1239-5
神经肽和受体
B-100-PS型
受体和通道分子
2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话：PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
B-200-PS型
PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
PE-1253-100型
TR-100-FJ
BL-005-500型
神经内分泌
M-1728-100型
DNA损伤与修复
PE-001-100型
PE-1608-1型
TR-400-AG公司
PE-1243-25
细胞标记
PE-1608-5型
TR-100-FJT
自噬
PE-1242-2型
其他神经元
神经递质和酶
抗氧化酶
神经元标志物
标记抗体
BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
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