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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Rabbit antibody to Tyrosine Hydroxylase (TH): IgG/R-1645-500/500 µg
商品详细Biosensis/Rabbit antibody to Tyrosine Hydroxylase (TH): IgG/R-1645-500/500 µg
Biosensis/Rabbit antibody to Tyrosine Hydroxylase (TH): IgG/R-1645-500/500 µg
Biosensis/Rabbit antibody to Tyrosine Hydroxylase (TH): IgG/R-1645-500/500 µg
商品编号: R-1645-500
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionTyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamine,epinephrineandnorepinephrine.ThereforetheregulationoftheTHenzymerepresentsthecentralmeansforcontrollingthesynthesisoftheseimportantcatecholamines.FUNCTION:Playsanimportantroleinthephysiologyofadrenergicneurons.CATALYTICACTIVITY:L-tyrosine+tetrahydrobiopterin+O2=3,4-dihydroxy-L-phenylalanine+4a-hydroxytetrahydrobiopterin.COFACTOR:Fe(2+)ion.ENZYMEREGULATION:Phosphorylationleadstoanincreaseinthecatalyticactivity.PATHWAY:Catecholaminebiosynthesis;firststep.SUBUNIT:Homotetramer.PTM:Invitro,phosphorylationofSer-19increasestherateofSer-40phosphorylation,whichresultsinenzymeopeningandactivation.SIMILARITY:Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.ThepresenceofdifferentDNAsequencesattheTHlocusconferssusceptibilitytovariousdisordersofthebrainincludingmanic-depressionandschizophrenia.Parkinson"sdiseaseisalsoconsideredaTHdeficiencyaslowdopaminelevelsareaconsistentneuRochemicalabnormality.
RelatedproductsR-118-100.R-1645-500istheProteinG-purifiedversionofR-118-100(wholeserum).
BatchNo.Seeproductlabel
Unitsize500µg
AntigenAsyntheticpeptide(PRFIGRRQSLIEDARK)aspartofhumanTyrosineHydroxylase(63-78)conjugatedtoKLHhasbeenusedastheimmunogen.ThepeptideishomologouswiththecorrespondingsequencederivedfromTHproteininrat(31-47).
OtherNamesTH;Tyrosinehydroxylase;Tyrosine3-monooxygenase;L-tyrosinehydroxylase;Tyrosine3-hydroxylase
AccessionP04177TY3H_RAT;
ProducedinRabbit
PurityProteinGpurified
ApplicationsIHC.Aconcentrationof4-10µg/mLisrecommendedforthisapplication.ThisisasuperbantibodyfordetectionoftyrosinehydroxylasecontainingneuronsexhibitinganintenselabellingwithanegligIBLebackground.Thisantiserumhasprovenextremelyusefulforstainingofcatecholaminergicneurons.Itstainsnicelyandintenselydendriticprocessesandfinenerveterminals.Werecommendmouseorratbraincontainingcatecholaminergicneuronsasapositivecontrolforthisantibody,forexamplebrainstemorstriatum.Westernblotting:Aconcentrationof5-15µg/mLisrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityIHConbrainshowsapatternofstainingspecificforTHcontainingneurons.
Cross-reactivityThisantibodyisknowntoreactwithrat,mouseandguineapig.Crossreactivitywithotherspecieshasnotyetbeentested.
BlastitIfyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis.
FormLyophilisedfromPBS,pH7.4,withoutpreservatives.
AppearanceWhitepowder
ReconstitutionReconstitutein500µLofsterilewater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionkeepaliquotsat-20ºCforahigherstABIlity,andat4ºCwithanappropriateantibacterialagent.Avoidrepetitivefreeze/thawcycles.
ExpiryDate12monthsafterpurchaseifunopened.
SpecificReferences1.PierreS.R.,LemmensM.A.,Figueiredo-PereiraM.E.(2009)SubchronicinfusionoftheproductofinflammationprostaglandinJ2modelssporADIcParkinson"sdiseaseinmiceJNeuroinflammation.Jul25;6:18
2.TakeokaA.etal(2010)Noradrenergicinnervationoftheratspinalcordcaudaltoacompletespinalcordtransection:effectsofolfactoryensheathinggliaJExpNeurol.2010Mar;222(1):59-69.
3.BrownR.E.etal(2008)CharacterizationofGABAergicneuronsinrapid-eye-movementsleepcontrollingregionsofthebrainstemreticularformationinGAD67-greenfluorescentproteinknock-inmice.EurJNeurosci.2008Jan;27(2):352-63.
4.BisemNJetal(2012)MappingofFGF1intheMedullaOblongataofMacacafascicularis.ActaHistochemCytochem.2012Dec26;45(6):325-34.
References1.Mallett,J.TrendsinPharmacologicalScience.17(4):129-135,1996.
2.Haavik,J.etal.Mol.NeuroBIOLOGy16(3):285-309,1998.
3.LewisDA,etal,Neuroscience54:477,1993
4.KumerS.C.etal.JournalofNeurochemistry,67(2):443-462,1996.
5.Haycock,J.Anal.Biochemistry181:259-266,1989.
6.Haycock,J.Anal.Biochemistry208:397-399,1993.
7.Renfroe,J.B.,etal.BrainRes.Bull.13:109-126,1984.
8.Xu,Zeta.lNeurosci.82(3):727,1998
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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