生物传感器/兔酪氨酸羟化酶抗体(TH;32-47):亲和纯化/R-148-50/50µg
商品编号:
R-148-50
品牌:
Biosensis inc
市场价:
¥4540.00
美元价:
2724.00
产品分类:
功能性抗体
公司分类:
Functional_antibody
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Description | Tyrosinehydroxylase(TH)istherate-limitingenzymeinthesynthesisofthecatecholaminesdopamine,epinephrineandnorepinephrine.ThereforetheregulationoftheTHenzymerepresentsthecentralmeansforcontrollingthesynthesisoftheseimportantcatecholamines.FUNCTION:Playsanimportantroleinthephysiologyofadrenergicneurons.CATALYTICACTIVITY:L-tyrosine+tetrahydrobiopterin+O2=3,4-dihydroxy-L-phenylalanine+4a-hydroxytetrahydrobiopterin.COFACTOR:Fe(2+)ion.ENZYMEREGULATION:Phosphorylationleadstoanincreaseinthecatalyticactivity.PATHWAY:Catecholaminebiosynthesis;firststep.SUBUNIT:Homotetramer.PTM:Invitro,phosphorylationofSer-19increasestherateofSer-40phosphorylation,whichresultsinenzymeopeningandactivation.SIMILARITY:Belongstothebiopterin-dependentaromaticaminoacidhydroxylasefamily.ThepresenceofdifferentDNAsequencesattheTHlocusconferssusceptibilitytovariousdisordersofthebrainincludingmanic-depressionandschizophrenia.Parkinson"sdiseaseisalsoconsideredaTHdeficiencyaslowdopaminelevelsareaconsistentneuRochemicalabnormality. |
Unitsize | 50µg |
Antigen | Asyntheticpeptide(SPRFIGRRQSLIEDARK)aspartofhumanTyrosineHydroxylase(aa:32-47)conjugatedtoKLH |
OtherNames | Tyrosinehydroxylase;Tyrosine3-monooxygenase;L-tyrosinehydroxylase;Tyrosine3-hydroxylase; |
Accession | TY3H_RAT |
Producedin | Rabbit |
Purity | Affinitypurified |
Applications | IHC.Adilutionof0.5-1µg/mlisrecommendedforthisapplication.ThisisasuperbantibodyfordetectionoftyrosinehydroxylasecontainingneuronsexhibitinganintenselabellingwithanegligIBLebackground.Thisantiserumhasprovenextremelyusefulforstainingofcatecholaminergicneurons.Itstainsnicelyandintenselydendriticprocessesandfinenerveterminals.Highlyrecommended.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
Specificity | IHConbrainshowsapatternofstainingspecificforTHcontainingneurons. |
Cross-reactivity | Thisantibodyisknowntoreactwithrat.Crossreactivitywithotherspecieshasnotyetbeentested. |
Blastit | Ifyouwouldliketousethisproductinanotherspeciesotherthanthosespecifiedhere,ortoseethesharedidentitybetweentheimmunogenusedhereindifferentspeicesand/orothermolecules,simplycopytheimmunogen(fromtheImmunogenfield)andpasteitHEREandblast/formatit.Notethat,antiseraraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptideie,onlyonesingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.Regardless,youcanalwayscontactusifyouneedassistancewiththis. |
Form | Lyophilised |
Reconstitution | Reconstitutein50µlofsterilewater.Centrifugetoremoveanyinsolublematerial. |
Storage | Afterreconstitutionkeepaliquotsat-20°CforahigherstABIlity,andat4°Cwithanappropriateantibacterialagent.Glycerol(1:1)maybeaddedforanadditionalstability.Avoidrepetitivefreeze/thawcycles. |
References | 1.Mallett,J.TrendsinPharmacologicalScience.17(4):129-135,1996. 2.Haavik,J.etal.Mol.NeuroBIOLOGy16(3):285-309,1998. 3.LewisDA,etal,Neuroscience54:477-,1993 4.KumerS.C.etal.JournalofNeurochemistry,67(2):443-462,1996. 5.Haycock,J.Anal.Biochemistry181:259-266,1989. 6.Haycock,J.Anal.Biochemistry208:397-399,1993. 7.Renfroe,J.B.,etal.BrainRes.Bull.13:109-126,1984.8.Xu,Zeta.lNeurosci.82(3):727,1998 |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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