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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/抗人p75NTR[ME20.4]-FITC/M-017-100-FT/100µg的小鼠单克隆抗体
商品详细Biosensis/抗人p75NTR[ME20.4]-FITC/M-017-100-FT/100µg的小鼠单克隆抗体
Biosensis/抗人p75NTR[ME20.4]-FITC/M-017-100-FT/100µg的小鼠单克隆抗体
Biosensis/抗人p75NTR[ME20.4]-FITC/M-017-100-FT/100µg的小鼠单克隆抗体
商品编号: M-017-100-FT
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionNervegrowthfactorreceptor(NGFR)isalsoreferredtoasp75(NTR)duetoitsmolecularmassanditsABIlitytobindatlowaffinitynotonlyNGF(see162030),butalsootherneurotrophins,includingbrain-derivedneurotrophicfactor(BDNF;113505),neurotrophin-3(NTF3;162660),andneurotrophin-4/5(NTF5;162662).Atthetimeofitsdiscovery,NGFRwasconsideredauniquetypeofprotein.Subsequently,however,alargesuperfamilyoftumornecrosisfactorreceptorswerefoundtosharetheoverallstructureofNGFR(4extracellularligand-binding,cysteine-richrepeats,orCRs,andsignalingthroughassociationwith,ordisassociationfrom,cytoplasmicinteractors).TheidentificationofthissuperfamilyhelpedelucidatesomeoftheBIOLOGicfunctionsofNGFR,includingitsultimateinvolvementinthenuclearfactorkappa-B(NFKB;see164011)andapoptosispathways.Asamonomer,NGFRbindsNGFwithlowaffinity.Higheraffinitybindingisachievedbyassociationwithhighermolecularmass,low-affinityneurotrophinreceptors,namelythetropomyosinreceptorkinases,TRKA(NTRK1;191315),TRKB(NTRK2;600456),andTRKC(NTRK3;191316).TRKA,TRKB,andTRKCarespecificforor"preferredby"NGF,NTF5andBDNF,andNTF3,respectively.NTF3alsobindstoTRKAandTRKB,butwithsignificantlyloweraffinity.
RelatedproductsM-1763-100-FT,Non-specificControlIgG,cloneX63(Monoclonal)-FITC
BatchNo.Seeproductlabel
Unitsize100µg
AntigenThep75NTRantibodywasderivedfromimmunizationofmicewithhumanWM245melanomacells.
CloneME20.4
OtherNamesLow-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR
AccessionTNR16_HUMAN
ProducedinMouse
PurityImmunoglobulin(IgG1)waspurifiedusingProteinGcolumn(AmershamPharmacia),polishedwithSephacryl200HR(AmershamPharmacia)inPBS.TheantibodywasthenconjugatedtoFluoresceinisomer1(FITC,Sigma).Aminimumfluorescein:proteinratioof3:1isguaranteed.TheconjugatewaspurifiedviagelfiltrationusingaG25finegraingelin10mMTris/50mMNaClsolution.
ApplicationsThisantibodyisrecommendedforuseinimmunohistochemistry,immunofluorescence,flowcytometryandNGFreceptorp75dynamics.Forimmunohistochemistryaconcentrationof2µg/mlisrecommended.NotappropriateforWesternBlots.ForFACSaconcentrationof20µg/mlisrecommendedandfor1siteELISAatleasta1in5000dilution.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThisantibodyrecognisesp75NTR(lowaffinityneurotrophinreceptor)
Cross-reactivityReactswithhuman,cat,dog,pig,rabbitandsheep.Doesnotreactwithratormouse.
FormLiquid.10mMTris,50mMNaCl
StorageTheantibodyconjugatecanbestoredat4ºCforupto4monthswiththeadditionofappropriateantibacterialagent.
ExpiryDateFourmonthsafterpurchase
References1.WalkerBRetal.(2007)BehavBrainRes176:109-20
2.RossA.H.etal.(1984)Characterizationofnervegrowthfactorreceptorinneuralcresttumorsusingmonoclonalantibodies.ProcNatlAcadSciUSA.1984Nov;81(21):6681-5.ORIGINALPAPER
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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