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当前位置: 首页 > 产品中心 > Other_recombinant_protein > Biosensis/小鼠神经生长因子(2.5S)蛋白质/PE-019-500/500µg
商品详细Biosensis/小鼠神经生长因子(2.5S)蛋白质/PE-019-500/500µg
Biosensis/小鼠神经生长因子(2.5S)蛋白质/PE-019-500/500µg
Biosensis/小鼠神经生长因子(2.5S)蛋白质/PE-019-500/500µg
商品编号: PE-019-500
品牌: Biosensis inc
市场价: ¥13940.00
美元价: 8364.00
产地: 美国(厂家直采)
公司:
产品分类: 其它重组蛋白
公司分类: Other_recombinant_protein
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionMouseNGF(2.5S)wasisolatedfrommousesuBMAxillaryglandsbymethodofMobleyetal(1976)andisaformofbeta-NGFthathasidenticalBIOLOGicalproperties.NGFisknowntoregulatethesurvivalanddevelopmentofcertainsympatheticandsensoryneurons.Itisadimerwith2identicalpolypeptidechainsanddimericmolecularweightofapproximately26,500Da.IsolationandpurificationofNGFfrommousesubmaxillaryglandsyieldspreparationsofNGF(2.5S)withidenticalbiologicalactivitybutwithcleavagesattheaminoterminus(withthelossof8aminoacids)and/oratthecarboxy-terminus(withthelossofarginine).Thesepreparationsarenamed2.5NGF(seereferencebelow).
BatchNo.Seeproductlabel
Unitsize500µg
OtherNamesmouseNGF;betaNGF
AccessionNGF_MOUSE
ProducedinMouse
MolecularWeightThehomodimerhasamolecularweightof~26KDaandthemonomer~13KDaasconfirmedbySDSPAGE.
PurityGreaterthan90%(asdeterminedbySDSelectrophoresis)
Biol.activityTestedbystimulationof8dayoldchickembryodorsalrootganglia.
FormLyophilisedfrom0.1MaceticacidunderdryN2withvacuum.Appearsasnear-whitepowder.Solutionsterilefilteredpriortodrying.
ReconstitutionReconstitutewiththeadditionof500µlsterilewaterorphosphatebufferedsalineanddivideintosingleusealiquots.Flashfreezealiquotsinadryiceethanolbathandstoreat-20Cto-70Cforbestresults.
StorageShortterm:-20°C.Avoidrepeatedfreezingandthawing.Longerterm-70C.Seeexpirationdateforshelf-lifeestimates,actualtimesmayvarydependinguponexperimentalconditionsandlaboratoryhandling.
ExpiryDate12monthsuponpurchasefordrymaterial.Hydratedmaterial,5daysat4C,30days-20C,60days-70CforhighestactivityAvoidrepeatedfreezingandthawing.Useinsulatedstoragecontainersforbestresults.
SpecificReferencesCarterDRetal(2016)GlutathionebiosynthesisisupregulatedattheinitiationofMYCN-drivenneuroblastomatumOrigenesis.MolOncol.2016Jun;10(6):866-78.

GoodhillGJetal(2015)Thedynamicsofgrowthconemorphology.BMCBiol.2015Feb;13(10).

CoulsonEJetal(2015)Neurotrophin-tyrosinekinasereceptorsignaling.UnitedStatesPatentApplication20150344535.

Gearing,DPetal(2013)Afullycaninisedanti-NGFmonoclonalantibodyforpainreliefindogs.BMCVetRes.9:226.

MatusicaDetal(2013)Anintracellulardomainfragmentofthep75neurotrophinreceptor(p75(NTR))enhancestropomyosinreceptorkinaseA(TrkA)receptorfunction.JBiolChem.2013Apr19;288(16):11144-54.

YuanJetal(2013)Optimalityandsaturationinaxonalchemotaxis.NeuralComput.2013Apr;25(4):833-53.

SykesAMetal(2012)Theeffectsoftransmembranesequenceanddimerizationoncleavageofthep75neurotrophinreceptorbyγ-secretase.JBiolChem.2012Dec21;287(52):43810-24.

E.M.Forbesetal(2012)CalciumandcAMPLevelsInteracttoDetermineAttractionversusRepulsioninAxonGuidance.Neuron.2012May10;74(3):490-503.
GeneralReferencesMobleyWC,SchenkerA,ShooterEM(1976)Biochemistry.15(25)pp.5543-52.
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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