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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Rabbit polyclonal antibody to NT3 (174-189): Affinity purified/R-1016-100/100 µg
商品详细Biosensis/Rabbit polyclonal antibody to NT3 (174-189): Affinity purified/R-1016-100/100 µg
Biosensis/Rabbit polyclonal antibody to NT3 (174-189): Affinity purified/R-1016-100/100 µg
Biosensis/Rabbit polyclonal antibody to NT3 (174-189): Affinity purified/R-1016-100/100 µg
商品编号: R-1016-100
品牌: Biosensis inc
市场价: ¥6540.00
美元价: 3924.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionNT3isamemberoftheneurotrophinfamily,thatcontrolssurvivalanddifferentiationofvisceralandproprioceptivesensoryneurons.NT3iscloselyrelatedtobothNGFandBDNF.Itmaybeinvolvedinthemaintenanceoftheadultnervoussystem,andmayaffectdevelopmentofneuronsintheembryowhenitisexpressedinhumanplacenta.NT3-deficientmicegeneratedbygenetargetingdisplayseveremovementdefectsofthelimbs.Thematurepeptideofthisproteinisidenticalinallmammalsexaminedincludinghuman,pig,ratandmouse.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily.
BatchNo.Seeproductlabel
Unitsize100µg
AntigenAsyntheticpeptide(TVLGEIKTGNSPVKQY)correspondingtoaminoacidsequence174-189fromhumanneurotrophin3(NT3).
OtherNamesNT-3;NTF3;Neurotrophin-3;HDNF;Nervegrowthfactor2;NGF-2;
AccessionP20783NTF3_HUMAN
ProducedinRabbit
PurityAffinitypurifiedonantigencolumn
ApplicationsImmunohistochemistry(IHC)andWesternBlotting(WB).Aconcentrationof0.1-0.5µg/mlisrecommendedforWB.HumanNT3hasapredictedlengthof257aminoacidsandMWof29kDa.Aconcentrationof0.5-1.0µg/mlisrecommendedtodetectNT3inparaffinembeddedtissues.Antigenretrievalbyboilingtheparaffin/formalinsectionin10mMcitratebuffer,pH6.0for20minutesisrequired.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityThespecificityofthisantibodyhasbeenconfirmedbyWBandIHCagainsttheantigen.
Cross-reactivityHuman;mouse;rat;
BlastitIfyouwishtousethisproductinaspeciesotherthanthosespecifiedhereandwanttodeterminetheaminoacidsequencehomologybetweentheimmunogenandthecorrespondingproteininthatspecies,simplycopytheaminoacidsequenceforthisimmunogenandtestitontheBLASTdatabasethatyoucanaccessthroughthelinkbelow.Pleasenotethatantibodiesraisedagainstsyntheticpeptidesarequiteoftenveryspecificforthatpeptide.Whatthismeansinpractiseisthatasingleaminoaciddifferencemaybeenoughtorestrictthespecificitytoaparticularmolecule.TheBLASTdatabasecanalsobeusedtocompareanyaminoacidsequencebetweenspeciesandbetweenprotein.ThefullaminoacidsequenceofthetargetproteincanbefoundbyfollowingthelinkintheAccessionfield.YoucanthensearchanyaminoacidsequenceinthetargetproteinusingtheBLASTdatabase.Whenselectingasequencetosearch,pleasechosecarefully,asthesequenceyouchosemaybeaprecursorratherthanthematureproteinforexample.
FormLyophilized.Eachvialcontains5mgBSA,0.9mgNaCl,0.2mgNa2HPO4,0.05mgThimerosal,0.05mgNaN3.
ReconstitutionAdd0.2mlofdistilledwaterwillyieldaconcentrationof500ug/ml.
StorageMaintainlyophilizedantibodyat2-8°C.Afterreconstitution,aliquotandstoreat-20°Cforupto6monthsforahigherstABIlity.Avoidfreeze-thawcycles
ExpiryDate12monthsafterpurchase(unopened).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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