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当前位置: 首页 > 产品中心 > Functional_antibody > Biosensis/Mouse monoclonal antibody to Heat Shock Protein 27 (HSP27)/M-1692-100/100 uL
商品详细Biosensis/Mouse monoclonal antibody to Heat Shock Protein 27 (HSP27)/M-1692-100/100 uL
Biosensis/Mouse monoclonal antibody to Heat Shock Protein 27 (HSP27)/M-1692-100/100 uL
Biosensis/Mouse monoclonal antibody to Heat Shock Protein 27 (HSP27)/M-1692-100/100 uL
商品编号: M-1692-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 功能性抗体
公司分类: Functional_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionTheheatshockproteinswerediscovered,asthenamesuggests,sincetheyareheavilyupregulatedwhencellsarestressedbytemperaturesabovethenormalphysiologicalrange.Theyareexpressedinunstressedcellsalsoandhaveanormalfunctionaschaperones,helpingotherproteinstofoldcorrectly,andarerequiredinmuchgreateramountsifthecellortissueisstressedbyheat.Theincreasedlevelsaregeneratedtranscriptionallyundertheinfluenceofapowerfultranscriptionfactor,theheatshockfactor1(HSF1).ThedifferentheatshockproteinswereoriginallynamedbasedontheirSDS-PAGEmobility,soHSP27hasanapparentmolecularweightof27kDa.Itisanabundantproteinevenundernon-stressconditionsandfrequentlyshowsupasamajorspoton2dimensionalgelsofcellsortissues.Itisknowntoassociatewithavarietyofotherproteinssuchasactin,intermediatefilamentsubunitsandubiquitinandisfoundbothinthecytoplasmandthenucleusofcells.HSP27canbecomeheavilyphosphorylatedundertheinfluenceofmultipleproteinkinasesparticularlyasaresultofactivationofthep38/SAPKpathway.Upregulationofthisproteinisprotectiveagainstneurodegenerativediseasesatleastincertainmousemodels(1).PointmutationsintheHSP27geneareassociatedwithtwoneurologicaldiseases,Charcot-Marie-Toothdiseasetype2FanddistalhereditarymotorneuropathyIIB(2).Thesediseasesareassociatedwithaxonallossapparentlyfollowingdefectsinthetransportofneurofilaments.
Unitsize100uL
AntigenHSP27
IsotypeIgG1
ProducedinMouse
PurityIgG
ApplicationsWesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:5,000-1:10,000isrecommendedforWB.Adilutionof1:500-1:1,000isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityTheantibodyreactswitha27kDabandbyWesternblotonacrudeextractfromHeLacells.Ithasalsobeenusedsuccessfullyforimmunocytochemistry.
SpeciesAgainstHuman,rat,mouse,porcineandbovine.Itisexpectedthatitwillworkonothermammaltissues.
AntibodyAgainstHeatShockProtein27(HSP27)
FormLyophilisedwith5%trehalose
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilisedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
GeneralReferences1.Wyttenbach,Aetal.Heatshockprotein27preventscellularpolyglutaminetoxicityandsuppressestheincreaseofreactiveoxygenspeciescausedbyhuntingtin.Hum.Molec.Genet.11:1137-1151(2002).2.Evgrafov,OVetal.Mutantsmallheat-shockprotein27causesaxonalCharcot-Marie-Toothdiseaseanddistalhereditarymotorneuropathy.NatureGenet.36:602-606(2004).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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