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Biosensis/Mouse monoclonal antibody to rat capsaicin receptor (VR1, TRPV1, 819-838), [Clone BS397]: IgG/M-1714-100/100 µg
![Biosensis/Mouse monoclonal antibody to rat capsaicin receptor (VR1, TRPV1, 819-838), [Clone BS397]: IgG/M-1714-100/100 µg](https://www.ebiomall.cn/images/biosensis/201712/M-1714-100_Merged.jpg)
Biosensis/Mouse monoclonal antibody to rat capsaicin receptor (VR1, TRPV1, 819-838), [Clone BS397]: IgG/M-1714-100/100 µg
商品编号：
M-1714-100
品牌：
Biosensis inc
市场价：
¥5940.00
美元价：
3564.00
产品分类：
功能性抗体
公司分类：
Functional_antibody
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Description | Thecapsaicinreceptor(VR1,TRPV1)isaligand-activatednon-selectivecalciumpermeantcationchannelinvolvedindetectionofnoxiouschemicalandthermalstimuli.Thereceptorseemstomediateprotoninfluxandmaybeinvolvedinintracellularacidosisinnociceptiveneurons.Itisinvolvedinmediationofinflammatorypainandhyperalgesia.Sensitizedbyaphosphatidylinositolsecondmessengersystemactivatedbyreceptortyrosinekinases,whichinvolvesPKCisozymesandPCL.Activationbyvanilloids,likecapsaicin,andtemperatureshigherthan42degreesCelsius,exhibitsatime-andCa2+-dependentoutwardrectification,followedbyalong-lastingrefractorystate.MildextracellularacidicpH(6.5)potentiateschannelactivationbynoxiousheatandvanilloids,whereasacidicconditions(pHlessthan6)directlyactivatethechannel.Canbeactivatedbyendogenouscompounds,including12-hydroperoxytetraenoicacidandbradykinin.ActsasionotropicendocannABInoidreceptorwithcentralneuromodulatoryeffects.Triggersaformoflong-termdepression(TRPV1-LTD)mediatedbytheendocannabinoidanandamineinthehippocampusandnucleusaccumbensbyaffectingAMPAreceptorsendocytosis(Ref:uniprot.org). |
| Unitsize | 100µg |
| Antigen | Asyntheticpeptide(C-GSLKPEDAEVFKDSMVPGEK)asapartoftheC-terminalratVR1protein(aa:819-838)hasbeenusedastheimmunogen. |
| Sequence | C-GSLKPEDAEVFKDSMVPGEK;aa819-838ratVR1 |
| AntigenLocation | C-terminal |
| AntigenLength | 20aminoacids |
| AntibodyType | MousemonoclonalIgG |
| Isotype | IgG2b,k-lightchain |
| Clone | BS397 |
| OtherNames | VR1;TransientreceptorpotentialcationchannelsubfamilyVmember1;TrpV1;osm-9-likeTRPchannel1;OTRPC1;Vanilloidreceptor1;Capsaicinreceptor;VR-1 |
| Accession | Uniprot:O35433;TRPV1_RAT |
| Producedin | Mouse |
| MolecularWeight | Monomer90-100kDainmousebrainextracts;dimer180-200kDacanbeobservedundersomeconditions |
| Purity | ProteinGpurifiedmouseimmunoglobulin |
| Applications | FlowCytometry:2µg/10^6cells. Westernblotting:0.5-2µg/mL,SDS-PAGEonBis-Trisgel4-12%,5%beta-mercaptoethanol,primaryantibodyO/Nincubationin5%skimmilk/TBST.Secondaryisanti-mouse-HRP,1/6000dilution,2hatroomtemperature.BlotdevelopedonLI-COR®C-DiGit®lotScanner. IHC:FrozenorPEGembeddedtissuestested(PEGembedding,seeKlosenPetal(1993)JHistochemCytochem.41(3):455-63).Conditionstested:1-10µg/mLinPBS,48hours,followedbydetectionviadirectlyconjugatedfluorescentanti-mousesecondary.Antibodynotyettestedonparaffinembeddedsections.Otherimmunohistochemistrymethodsnotyettestedbutareexpectedtobereactive. ICC:4%formaldehydefixedcellstested;requirespermeabilizationstepasantigenepitopeisintracellular.Suggestedprimaryantibodyconcentration:1-2µg/mL. Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser. |
| Specificity | Antibodyisspecificforrat/mouseVR1proteininwesternsandimmunofluorescentimmunohistochemistryonmousePEGfixedDRGtissues.Pre-absorptionwithimmunogenobliteratespositivestaining.Crossreactivitywithothernon-VR1proteinsisminimal;crossreactivitywithVR1fromotherspeciesnotyettested. |
| SpeciesAgainst | Rat/mouse,otherspeciesnotyettested |
| AntibodyAgainst | RatVR1 |
| Cross-reactivity | ThisantibodycloneisknowntoreactwithratandmouseTrpV1.Itispredictedtoreactwithguineapigduetosequencehomology. |
| Form | LyophilizedfromPBS,pH7.4with3%trehalose. |
| Appearance | Drypowder |
| Reconstitution | Reconstitutein100µLofsterilewater.Centrifugetoremoveanyinsolublematerial.Finalbuffercontainsnopreservativesbutwillcontain3%trehaloseandbuffersalts. |
| Storage | Afterreconstitutiondivideintoaliquotsandstoreat-20ºCforahigherstability.Antibodycontainsnopreservatives.Storageat4ºCwithanappropriateantibacterialagent.USESterilemethods.HighestpurityGlycerol(1:1)maybeaddedforanadditionalstabilitywhenstoredatrefrigeratedorfreezingtemperatures.Avoidrepetitivefreeze/thawcycles. |
| ExpiryDate | 12monthsafterpurchaseifunopened. |
| GeneralReferences | PengH.Y.(2008)TRPV1mediatestheuterinecapsaicin-inducedNMDANR2B-dependentcross-organreflexsensitizationinanesthetizedrats.AmJPhysiolRenalPhysiol.Nov;295(5):F1324-35. |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
TR-100-BG型
S-1754-500型
神经营养因子
糖尿病与肥胖
细胞内贩运
电话：PE-1239-5
神经肽和受体
B-100-PS型
受体和通道分子
2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话：PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
B-200-PS型
PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
PE-1253-100型
TR-100-FJ
BL-005-500型
神经内分泌
M-1728-100型
DNA损伤与修复
PE-001-100型
PE-1608-1型
TR-400-AG公司
PE-1243-25
细胞标记
PE-1608-5型
TR-100-FJT
自噬
PE-1242-2型
其他神经元
神经递质和酶
抗氧化酶
神经元标志物
标记抗体
BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
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