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当前位置: 首页 > 产品中心 > Tag_antibody > Biosensis/小鼠抗mCherry单克隆抗体:IgG/M-1653-100/1 Ea
商品详细Biosensis/小鼠抗mCherry单克隆抗体:IgG/M-1653-100/1 Ea
Biosensis/小鼠抗mCherry单克隆抗体:IgG/M-1653-100/1 Ea
Biosensis/小鼠抗mCherry单克隆抗体:IgG/M-1653-100/1 Ea
商品编号: M-1653-100
品牌: Biosensis inc
市场价: ¥5940.00
美元价: 3564.00
产地: 美国(厂家直采)
公司:
产品分类: 标签抗体
公司分类: Tag_antibody
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionmCherryisanengineeredderivativeofoneofafamilyofproteinsoriginallyisolatedfromCnidarians(jellyfish,seaanemonesandcorals).ThemCherryproteinwasderivedfromDsRed,aredfluorescentproteinfromso-calleddisccoralsofthegenusDiscosoma.DsRedisa223aminoacid~28kDaproteinsimilarinsizeandpropertiestoGFP,but,obviously,producesaredratherthanagreenFluorochrome.TheoriginalDsRedwasengineeredextensivelyintheTsienlabtopreventitfromformingtetramersanddimersandtomodifyandimprovethespectralproperties(1-3).TheresultingmonomericproteinisusefulforapplicationssuchasFörsterResonanceEnergyTransfer(FRET,alsoknownasFluorescenceResonanceEnergyTransfer).Severalfurthercyclesofmutation,directedmodificationandevolutionaryselectionproducedmCherry,whichismonomericandhasanexcitationmaximumat587nmandandemissionmaximumat610nm(4).
AntigenRecombinantfulllengthmCherryexpressedandpurifiedfromE.coli.
AntibodyTypeLiquidinPhosphateBufferedSaline.Theantibodyhasbeenpurifiedfromtissueculturesupernatant.
IsotypeIgG2a
ProducedinMouse
ApplicationsWesternBlotting(WB)andImmunocytochemistry(IC).Adilutionof1:1,000to1:2,000isrecommendedforWB.Adilutionof1:250to1:500isrecommendedforIC.Biosensisrecommendsoptimaldilutions/concentrationsshouldbedeterminedbytheenduser.
SpecificityTheantibodyreactswithabandat~28kDacorrespondingtointactfull-lengthmCherrybyWesternblotonHEK293cellstransfectedwithmCherryvector.Ithasalsobeenusedsuccessfullyforimmunocytochemistry.
SpeciesAgainstSpeciesindependent
AntibodyAgainstmCherry
FormLyophilized
AppearanceWhitepowder
ReconstitutionReconstituteinsteriledistilledwater.Centrifugetoremoveanyinsolublematerial.
StorageAfterreconstitutionoflyophilizedantibody,aliquotandstoreat-20°CforahigherstABIlity.Avoidfreeze-thawcycles.
ExpiryDate12monthsafterpurchase
GeneralReferences1.ChalfieM,TuY,EuskirchenG,WardWW,PrasherDCGreenfluorescentproteinasaMarkerforgeneexpression.Science.263:802-5(1994).2.BairdGS,ZachariasDA,TsienRY.Biochemistry,mutagenesis,andoligomerizationofDsRed,aredfluorescentproteinfromcoral.ProcNatlAcadSciUSA.97:11984-9(2000).3.GrossLA,BairdGS,HoffmanRC,BaldridgeKK,TsienRY.ThestructureofthechromophorewithinDsRed,aredfluorescentproteinfromcoral.ProcNatlAcadSciUSA.97:11990-5(2000).4.HeikalAA,HessST,BairdGS,TsienRY,WebbWW.Molecularspectroscopyanddynamicsofintrinsicallyfluorescentproteins:coralred(dsRed)andyellow(Citrine).ProcNatlAcadSciUSA.97:11996-2001(2000).5.ShanerNC,CampbellRE,SteinbachPA,GiepmansBN,PalmerAE,TsienRY.Improvedmonomericred,orangeandyellowfluorescentproteinsderivedfromDiscosomasp.redfluorescentprotein.NatureBiotechnology22:1567-1572(2004).
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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