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当前位置: 首页 > 产品中心 > Other_reagents > Biosensis/Phospho-Sure RTD神经细胞和软组织提取缓冲液/B-200-PS/200 mL
商品详细Biosensis/Phospho-Sure RTD神经细胞和软组织提取缓冲液/B-200-PS/200 mL
Biosensis/Phospho-Sure RTD神经细胞和软组织提取缓冲液/B-200-PS/200 mL
Biosensis/Phospho-Sure RTD神经细胞和软组织提取缓冲液/B-200-PS/200 mL
商品编号: B-200-PS
品牌: Biosensis inc
市场价: ¥2400.00
美元价: 1440.00
产地: 美国(厂家直采)
公司:
产品分类: 其他试剂
公司分类: Other_reagents
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
DescriptionDetergentstypicallypresentincelllysisbuffersarethoughttodisruptorganellesandcompartmentsandincreasetheexposureofsolublephospho-proteinstophosphatasesandproteasestherebyresultinginuncontrolleddephosphorylationandproteolysis.

Phospho-Sure™RTDNeuronalextractionbufferisoptimizedfortheextractionofphosphorylatedproteinsfromneuronalandothersofttissuestypes.Thebufferextractsthephosphoproteinsinanativestatewithouttheuseofharshdetergentsoroxidizers,anditisspeciallyformulatedtohelpmaintainphosphoproteinsandprotectthemfromdegradationbetterthantrADItionaldetergentbasedextractionbuffers.
BatchNo.Seeproductlabel
Unitsize200mL
OtherNamesPhosphosure
ApplicationsPleasedownloadtheprotocolbelowfordetailedinstructionsonhowtousePhospho-Sureinneuronalandothersofttissues.
FeaturedArticlesRefertothepublicationbySunejaetal(2006)wherethisbufferwasshowntoimproverecoveryofphospho-proteinssuchasERK1/2-PandSAP/JNK-Pinthedissectionandhomogenizationofguineapigbraintissue.
CommentsPhospho-Sure™RTD™isdesignedasbothanisolationandextractionbufferandforbestresultstheintacttissueshouldbebathedinice-coldPhospho-sure™RTD™bufferduringexcisionofthetissue.OncethedesiredtissuepieceisisolatedinPhospho-sure™RTD™itmaybetransferredtoasmallvolumeofice-coldPhospho-Sure™RTD™fordisruption.
FormPowdercontainingnopreservatives.PreparationofPhospho-­Sure™RTD™isverysimple.SimplyaddsterileddH2Odirectlytothepowderedsolutioninthesuppliedbottle,swirlbrieflytodissolvethechemicalsanduse.Thematerialcontainsnopreservativessosteriletechniqueshouldalwaysbeused.
AppearanceWhitepowder
ReconstitutionAdd186mlofsteriledoubledistilledwaterandmixbygentlyswirling,finalvolume200mlat20°C.
StorageThedry,unopenedcontainershouldbestoredatroomtemperatureinadryordesiccatedlocationprotectedfromlight.Donotstoreintherefrigeratorunlessmaterialisinadry,moisturefreeenvironment.Materialishydroscopicsooncethesealisbrokenitshouldbehydratedandnotresealedwhiledry.Oncehydrated,thebuffercanbestoredat4°Cforupto3months.Solutioncanbefrozenbutclumpingmayoccuruponthawingandisnotrecommended.
ExpiryDatePowderedformatcanbestoredupto12monthsafterpurchaseundercool,dryconditions.
SpecificReferences1.SunejaSK,MoZ,PotashnerSJ.(2006)Phospho-CREBandotherphospho-proteins:improvedrecoveryfrombraintissue.JNeurosciMethods.2006Jan30;150(2):238-41.
2.ElviraMass,DagmarWachten,AnnaC.Aschenbrenner,AndréVoelzmann,MichaelHochemail(2014)MurineCreld1ControlsCardiacDevelopmentthroughActivationofCalcineurin/NFATc1Signaling,DevelopmentalCellVolume28,Issue6,p711–726
BufferprotocolClick to download the protocolB-200-PS_as_at_March2013.pdf
MSDSClick to download the Material Safety Data SheetMSDS_B-100_B-200_as_at_Apr2014.pdf
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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