Biosensis/Apolipoprotein E/beta-Amyloid (ApoE/A beta) Complex ELISA Kit (1 Plate)/BEK-2224-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	BiosensisisproudtoofferthefirstcommerciallyavailableApoE/β-amyloid(ApoE/Aβ)complexELISAkit.AsaresultofextensivecollaborationwithDr.LaDuslaboratoryatUICandvalidationbyBiosensis,thisELISAcanbeusedtoaccuratelyandconsistentlymeasuretheextentofApoE/Aβcomplexintissueextractsandothersamples.TheBiosensisApoE/AβComplexELISAkitisasandwichELISAandconsistsofapre-coatedmousemonoclonalanti-Aβcaptureantibody,ahighlyvalidatedApoE/Aβcomplexstandardthatispre-formed,lyophilizedandreadyforreconstitution,abiotinylatedApoEdetectionantibody,andhorserADIshperoxidase(HRP)-conjugatedstreptavidinanddetectionreagent.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltothelevelofApoE/Aβcomplexpresentinsamplesandproteinstandards.Importantly,awell-characterizedanduniqueApoE/Aβcomplexisincludedasastandard.Thiscomplexispre-formedandlyophilized,requiringonlyreconstitutionwithassaydiluentpriortouse.Inordertoassessnon-specificApoEproteinbinding,eachkitincludesadditionalplatespre-coatedwithcontrolantibody. ThepurposeofthiskitistheinvitroqualitativemeasurementofApoE/AβcomplexesinbrainextractsandCSFsamplesfrombothtransgenicmiceandhumansorprimates,relativetoaknownApoE/Aβcomplexstandard,onlyifusedasdirected.Thiskithasnotbeentestedforothersampleapplications.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Refertotheproductlabel.
Specificity	Human.
Storage	Storeat4°C.
ExpiryDate	12monthsfrompurchase.
SpecificReferences	TaiLMetal.(2013)JBiolChem.288(8):5914-26 TaiLMetal.(2014)MolNeurodegen.9:2
GeneralReferences	Review: TaiLMetal(2015).APOE-modulatedAβ-inducedneuroinflammationinAlzheimer"sdisease:currentlandscape,noveldata,andfutureperspective.JNeuRochem.133(4):465-88.
Kitcomponents	TheELISAkitboxcontains2x96pre-coatedstripplates(1plateMOAB-2antibodycoated,1platecontrolantibodycoated),proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	0.1-6.25%
Kitprotocol	BEK-2224_as_at_Dec2016.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf