Biosensis/GDNF快速ELISA试剂盒:大鼠(1平板)/BEK-2230-1P/1 Ea
商品编号:
BEK-2230-1P
品牌:
Biosensis inc
市场价:
¥7960.00
美元价:
4776.00
产品分类:
夹心法ELISA
公司分类:
Sandwich_method_ELISA
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.comDescription | TheBiosensisGDNFRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofGDNFinlessthan4hoursincellculturesupernatants,celllysates,andserumonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularratserumsamples. | ThisELISAkitconsistsofapre-coatedanti-GDNFcaptureantibody,abiotinylatedanti-GDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofGDNFpresentinsamplesandproteinstandards. ThisGDNFELISAkitemploysarecombinantratGDNFstandardandisthereforedesignedtomeasuretheratformofGDNF.Notethattheantibodiesusedinthiskitcross-reactwithmouse,humanandguineapigGDNF. Thiskithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Relatedproducts | ForaccuratequantificationofGDNFinmouseandhumansamples,werecommendBEK-2229andBEK-2222,respectively!WhileallGDNFRapidTMELISAkitsusethesamecaptureanddetectionantibodies,theproteinstandardsdiffertoreflecttheiruseineitherhumanorrodentsamples.Notethatduetoahighdegreeofsequencehomology,GDNFfromotherspeciesmaybequantified. | BatchNo. | Refertotheproductlabel. | Antigen | GDNFisaglycosylated,disulfide-bondedhomodimermolecule.ItwasfirstdiscoveredasapotentsurvivalfactorformidbraindopaminergicneuronsandwasthenshowntorescuetheseneuronsinanimalmodelsofParkinson"sdisease.GDNFisabout100timesmoreefficientsurvivalfactorforspinalmotorneuronsthantheneurotrophins.FUNCTION:Neurotrophicfactorthatenhancessurvivalandmorphologicaldifferentiationofdopaminergicneuronsandincreasestheirhigh-affinitydopamineuptake.SUBUNIT:Homodimer;disulfide-linked.SUBCELLULARLOCATION:Secretedprotein.ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.DISEASE:DefectsinGDNFmaybeacauseofHirschsprungdisease(HSCR).InassociationwithmutationsofRETgene,defectsinGDNFmaybeinvolvedinHirschsprungdisease.Thisgeneticdisorderofneuralcrestdevelopmentischaracterizedbytheabsenceofintramuralganglioncellsinthehindgut,oftenresultinginintestinalobstruction.DISEASE:DefectsinGDNFareacauseofcongenitalcentralhypoventilationsyndrome(CCHS);alsoknownascongenitalfailureofautonomiccontrolorOndinecurse.CCHSisararedisordercharacterizedbyabnormalcontrolofrespirationintheabsenceofneuromuscularorlungdisease,oranidentifiablebrainstemlesion.AdeficiencyinautonomiccontrolofrespirationresultsininadequateornegligIBLeventilatoryandarousalresponsestohypercapniaandhypoxemia.SIMILARITY:BelongstotheTGF-betafamily.GDNFsubfamily. | OtherNames | Glialcellline-derivedneurotrophicfactor;Astrocyte-derivedtrophicfactor;ATF;GDNF | Accession | GDNF_RAT | Specificity | Rat.TheantibodiesusedinthiskitdetecthumanandmouseGDNF. | Theassayantibodiescross-reactwithguineapigGDNFasevidencedbymeasuringGDNFinguineapigserumwiththeHumanGDNFRapidTMELISAkit.AllBiosensisGDNFRapidTMELISAkitsusethesamecaptureanddetectionantibodies,andthusthisRatGDNFRapidTMELISAkitcanbeusedtoassayguineapigGDNFinserum.However,inabsenceofatrueguineapigGDNFproteinstandard,theHumanGDNFRapidTMELISAkitmaygivethemostaccurateestimationsofguineapigGDNFlevelsbasedonaminoacidsequencehomology. Cross-reactivity | Nocross-reactivitywasobservedforthefollowingproteinstestedat25ng/mLinassaybuffer:brain-derivedneurotrophicfactor(rhBDNF),nervegrowthfactor(rhNGF),neurotrophin-3(rhNT-3),rhNT-4/5,vascularendothelialgrowthfactor(rhVEGF),recombinanthumanNeurturin,ArteminandPersephin. | Storage | Storeat4°C. | ExpiryDate | 12monthsfrompurchase. | Kitcomponents | TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols. | Range | 7.8-500pg/mL | Sensitivity | Typicallimitofdetection(LOD)forrrGDNFis<5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10). | Kitprotocol | BEK-2230_as_at_Feb2017.pdf | MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf | 品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
TR-100-BG型
S-1754-500型
神经营养因子
糖尿病与肥胖
细胞内贩运
电话:PE-1239-5
神经肽和受体
B-100-PS型
受体和通道分子
2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话:PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
细胞周期调节器
S-1759-100号
PE-1606-100型
PE-1762-100型
B-200-PS型
PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
PE-1253-100型
TR-100-FJ
BL-005-500型
神经内分泌
M-1728-100型
DNA损伤与修复
PE-001-100型
PE-1608-1型
TR-400-AG公司
PE-1243-25
细胞标记
PE-1608-5型
TR-100-FJT
自噬
PE-1242-2型
其他神经元
神经递质和酶
抗氧化酶
神经元标志物
标记抗体
BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
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