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商品详细Biosensis/GDNF快速ELISA试剂盒:大鼠(1平板)/BEK-2230-1P/1 Ea
Biosensis/GDNF快速ELISA试剂盒:大鼠(1平板)/BEK-2230-1P/1 Ea
Biosensis/GDNF快速ELISA试剂盒:大鼠(1平板)/BEK-2230-1P/1 Ea
商品编号: BEK-2230-1P
品牌: Biosensis inc
市场价: ¥7960.00
美元价: 4776.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
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品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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DescriptionTheBiosensisGDNFRapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofGDNFinlessthan4hoursincellculturesupernatants,celllysates,andserumonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularratserumsamples.

ThisELISAkitconsistsofapre-coatedanti-GDNFcaptureantibody,abiotinylatedanti-GDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofGDNFpresentinsamplesandproteinstandards.

ThisGDNFELISAkitemploysarecombinantratGDNFstandardandisthereforedesignedtomeasuretheratformofGDNF.Notethattheantibodiesusedinthiskitcross-reactwithmouse,humanandguineapigGDNF.

Thiskithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
RelatedproductsForaccuratequantificationofGDNFinmouseandhumansamples,werecommendBEK-2229andBEK-2222,respectively!WhileallGDNFRapidTMELISAkitsusethesamecaptureanddetectionantibodies,theproteinstandardsdiffertoreflecttheiruseineitherhumanorrodentsamples.Notethatduetoahighdegreeofsequencehomology,GDNFfromotherspeciesmaybequantified.
BatchNo.Refertotheproductlabel.
AntigenGDNFisaglycosylated,disulfide-bondedhomodimermolecule.ItwasfirstdiscoveredasapotentsurvivalfactorformidbraindopaminergicneuronsandwasthenshowntorescuetheseneuronsinanimalmodelsofParkinson"sdisease.GDNFisabout100timesmoreefficientsurvivalfactorforspinalmotorneuronsthantheneurotrophins.FUNCTION:Neurotrophicfactorthatenhancessurvivalandmorphologicaldifferentiationofdopaminergicneuronsandincreasestheirhigh-affinitydopamineuptake.SUBUNIT:Homodimer;disulfide-linked.SUBCELLULARLOCATION:Secretedprotein.ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.DISEASE:DefectsinGDNFmaybeacauseofHirschsprungdisease(HSCR).InassociationwithmutationsofRETgene,defectsinGDNFmaybeinvolvedinHirschsprungdisease.Thisgeneticdisorderofneuralcrestdevelopmentischaracterizedbytheabsenceofintramuralganglioncellsinthehindgut,oftenresultinginintestinalobstruction.DISEASE:DefectsinGDNFareacauseofcongenitalcentralhypoventilationsyndrome(CCHS);alsoknownascongenitalfailureofautonomiccontrolorOndinecurse.CCHSisararedisordercharacterizedbyabnormalcontrolofrespirationintheabsenceofneuromuscularorlungdisease,oranidentifiablebrainstemlesion.AdeficiencyinautonomiccontrolofrespirationresultsininadequateornegligIBLeventilatoryandarousalresponsestohypercapniaandhypoxemia.SIMILARITY:BelongstotheTGF-betafamily.GDNFsubfamily.
OtherNamesGlialcellline-derivedneurotrophicfactor;Astrocyte-derivedtrophicfactor;ATF;GDNF
AccessionGDNF_RAT
SpecificityRat.TheantibodiesusedinthiskitdetecthumanandmouseGDNF.

Theassayantibodiescross-reactwithguineapigGDNFasevidencedbymeasuringGDNFinguineapigserumwiththeHumanGDNFRapidTMELISAkit.AllBiosensisGDNFRapidTMELISAkitsusethesamecaptureanddetectionantibodies,andthusthisRatGDNFRapidTMELISAkitcanbeusedtoassayguineapigGDNFinserum.However,inabsenceofatrueguineapigGDNFproteinstandard,theHumanGDNFRapidTMELISAkitmaygivethemostaccurateestimationsofguineapigGDNFlevelsbasedonaminoacidsequencehomology.
Cross-reactivityNocross-reactivitywasobservedforthefollowingproteinstestedat25ng/mLinassaybuffer:brain-derivedneurotrophicfactor(rhBDNF),nervegrowthfactor(rhNGF),neurotrophin-3(rhNT-3),rhNT-4/5,vascularendothelialgrowthfactor(rhVEGF),recombinanthumanNeurturin,ArteminandPersephin.
StorageStoreat4°C.
ExpiryDate12monthsfrompurchase.
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range7.8-500pg/mL
SensitivityTypicallimitofdetection(LOD)forrrGDNFis<5 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10).
KitprotocolClick to download the kit protocolBEK-2230_as_at_Feb2017.pdf
MSDSClick to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf