Biosensis/Human Interleukin 8 ELISA Kit (1 plate)/BEK-2044-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ThehumanInterleukin8(IL-8)KitisasandwichELISA.ThecaptureantibodyisamonoclonalhumanIL-8antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownIL-8concentrationareaddedtothesewellsandallowedtocomplexwiththeboundIL-8antibody.AbiotinylatedhumanIL-8antibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIL-8presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanIL-8insamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
OtherNames	IL-8;Interleukin-8;C-X-Cmotifchemokine8;Emoctakin;Granulocytechemotacticprotein1;GCP-1;Monocyte-derivedneutrophilchemotacticfactor;MDNCF;Monocyte-derivedneutrophil-activatingpeptide;MONAP;Neutrophil-activatingprotein;NAP-1;Protein3-10C;T-cellchemotacticfactor;IL8;
Accession	P10145IL8_HUMAN;
Specificity	HumanIL-8
Storage	Storeat4øC
SpecificReferences	WickströmK,Stavréus-EversA,VercauterenO,OlovssonM,EdelstamG(2016)"EffectofLignocaineonIL-6,IL-8,andMCP-1inPeritonealMacrophagesandEndometrioticStromalCells"ReproductiveSciences;[Epubaheadofprint].Application:Cellculturesupernatant.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	15.6pg/ml-1,000pg/mlforbodyfluids,tissuelysatesorcellculturesupernates.7.8pg/ml-500pg/mlforhumanseraandplasma.
Sensitivity	< 1 pg/ml
Kitprotocol	BEK-2044-1P_as_at_Apr2016_protocol.pdf