Biosensis/Human Soluble E-Selectin (sE-Selectin) ELISA Kit (1 plate)/BEK-2089-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ThehumanE-SelectinKitisasandwichELISA.ThecaptureantibodyisapolyclonalhumanE-Selectinantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownE-SelectinconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundE-Selectinantibody.AbiotinylatedhumanE-Selectinmonoclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofE-Selectinpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanE-Selectininsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
OtherNames	CD62antigen-likefamilymemberE;Endothelialleukocyteadhesionmolecule1;ELAM-1;Leukocyte-endothelialcelladhesionmolecule2;LECAM2;CD62E;SELE;ELAM1;
Accession	P16581LYAM2_HUMAN;
Specificity	HumanE-Selectin
Storage	Storeat4øC
SpecificReferences	OYBang,JWChung,SJKimetal.(2016)"Caveolin-1,Ringfingerprotein213,andendothelialfunctioninMoyamoyadisease."IntJStroke.[Epubaheadofprint].Application:ELISA,Species:Human. OYBang,JWChung,SRyoo,GJMoon,GMKimetal.(2015)"BrainMicroangiopathyandMacroangiopathyShareCommonRiskFactorsandBioMarkers"AtherosclerosisInpress.Application:ELISA,Species:Human,blood.Biomarker,intracranialatherosclerosis,smallarterydisease,Riskfactor,renalfunction,Endothelialfunction
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	62.5pg/ml-4,000pg/ml
Sensitivity	< 4 pg/ml
Kitprotocol	BEK-2089-1P_as_at_Mar2015_protocol.pdf