Biosensis/Human TNFα/Cachectin/TNFSF2 ELISA Kit (1 plate)/BEK-2100-1P/1 Ea-免疫在线蚂蚁淘旗下平台

cancer官网 cellgs清关 cellbiologics正品 cellntec代购 db_biotech报价 diagnostic采购 etonbio everest exbio ez GenWay中国转染试剂24765 Active Bio价格 SBI Cascade华东代理 JPT经销商 ACROBiosystems授权蚂蚁淘河南必第生物科技有限公司

免责声明：

本站有部分内容来自互联网，如无意中侵犯了某个媒体、公司、企业或个人等的知识产权，请来电或致函告之，
本网站将在规定时间内给予删除等相关处理。

扫描二维码关注官方微信

获取最新促销及产品信息

	Finda2platesizeofthisproducthere.Enquireaboutbulkdiscountsfortenormoreplates,atsales@Biosensis.com
Description	Tumornecrosisfactoralpha(TNF-alpha)isacytokinethatbelongstothetumornecrosisfactor(TNF)superfamily.TNF-alphaismainlysecretedbymacrophages.TNF-alphaisinvolvedintheregulationofmanyprocessessuchas¶ÿcellproliferation,differentiationandapoptosisandhasbeenimplicatedinavarietyofdiseasesincludingcancer.TNF-alphaisexpressedasasingle-passtypeIImembraneprotein.Asoluble,secretedformisderivedfromthemembraneformbyproteolyticprocessing. ThehumanTNF-alphaKitisasandwichELISA.ThecaptureantibodyisamonoclonalhumanTNF-alphaantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownTNF-alphaconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundTNF-alphaantibody.AbiotinylatedhumanTNF-alphapolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTNF-alphapresentinthesamples.ThepurposeofthiskitisforquantitativedetectionofhumanTNFαincellculturesupernatants,serum,andheparin,EDTAorcitratetreatedplasmasamplesonlywhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
Antigen	ThestandardusedinthisTNFalphaELISAisrecombinanthumanTNFalphaexpressedinE.coliandpurified.Theexactsequenceusedmapstothesolubleform,aminoacids77-233ofhumanTNFalpha.
Sequence	>sp\|P01375\|77-233VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
OtherNames	TNF-alpha;Tumornecrosisfactorligandsuperfamilymember2;TNF-a;Cachectin;TNF;TNFA;TNFSF2;
Accession	P01375TNFA_HUMAN;
Applications	ELISA
Specificity	HumanTNF-alpha
Storage	Storeat4øC
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	15.6pg/ml-1,000pg/mlforbodyfluids,tissuelysatesorcellculturesupernates.7.8pg/ml-500pg/mlforhumansera.
Sensitivity	< 1 pg/ml
Kitprotocol	BEK-2100-1P_as_at_Nov2017_protocol.pdf