Biosensis/LR3IGF-I Rapid ELISA Kit: Human (1 plate)/BEK-2233-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	HumanLR³insulin-likeGrowthFactor-I(LR³IGF-I)wasdevelopedbyGroPepBioreagents(www.gropep.com)specificallyforsupplementationofmammaliancellculturetosupportthesurvivalandproliferationofcells.ItisengineeredtohaveahigherBIOLOGicalpotencythannativeIGF-IorIGF-IIandhasseveraladvantagesoverrecombinantinsulin.SupplementationofcellcultureswithLR³IGF-Iatamuchlowerconcentrationresultsinequivalentorbetterproductivitythansupplementationwithstandardconcentrationsofinsulin.LR³IGF-IisbetterabletostimulatethetypeIIGFreceptorandthusinduceahigherlevelofactivationofintracellularsignallingmolecules,whichareresponsIBLeforpromotingcellsurvivalbyinhibitionofapoptosis. ThisLR³IGF-IRapidELISAkitcombinesGroPepsmanyyearsofexpertiseinthefieldofIGFresearchandBiosensisnewlyestablishedRapidELISAplatform.ThiscollaborationhasresultedinthenewLR³IGF-IRapidELISAkit,whichprovidesforthesensitive,specificandreliablequantificationofLR³IGF-Iproteininlessthan3hours! TheELISAkitconsistsofacompletesetofreagentsandpre-coatedplatetoallowimmediateassayofLR³IGF-Iinculturemedia.Includedinthekitaremousemonoclonalanti-LR³IGF-Icaptureantibodypre-coatedontoanELISAplate,standardLR³IGF-Iprotein,abiotinylatedanti-LR³IGF-IdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofLR³IGF-Ipresentinsamplesandthesuppliedproteinstandard. ThisLR³IGF-IRapid^TMELISAkithasbeendeveloped,optimizedandvalidatedtoquantifyLR³IGF-Iproteinincellculturemedium.ItislikelytobeusedtomeasureLR³IGF-Iinmediaandduringdownstreamprocessingofmediafollowingaproductioncycleandisnotintendedforotheruse.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Refertotheproductlabel.
Antigen	LR³IGF-Iisan83aminoacidanalogueofIGF-IcomprisingthecompletehumanIGF-IsequencewiththesubstitutionofanArgininefortheGlutamineatposition3,plusa13aminoacidextensionpeptideattheN-terminus.
OtherNames	HumanLR3insulin-likeGrowthFactor-I.
Accession	Q9NO10IGF1_HUMAN(unmodifiedprotein)
Specificity	Human
Cross-reactivity	TheassayisintendedforquantificationofLR³IGF-I.Cross-reactionwithhumanIGF-Iis32%.Cross-reactionwithhumanIGF-IIislessthan0.01%.
Storage	Storeat4°C
ExpiryDate	12monthsfrompurchase.
Kitcomponents	The ELISA kit box contains 1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range	3.9ng/mL-200ng/mL
Sensitivity	Typicallimitofdetection(LOD)forLR³IGF-Iis1ng/mLdeterminedas150%oftheblankvalue.
Kitprotocol	BEK-2233_as_at_May2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf