Biosensis/Mature BDNF Rapid ELISA Kit: Human, Mouse, Rat (1 plate)/BEK-2211-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TheBiosensisMatureBDNFRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofmatureBDNFinlessthan3hoursincellculturesupernatants,serum,plasma(citrateandEDTA),celllysates,humanmilkandbrainextractsonlyifusedasdirected,withasimplifiedprotocolandnolossofsensitivityorspecificity.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularbloodsamples.NotethataccuratequantificationofBDNFinhumanmilkrequiresasecretoryIgA(sIgA)blockerwhichcanbepurchasedseparately(BL-001-1250).FormeasurementofmatureBDNFinCSFsamples,pleasecontactusatsales@biosensis.com. ThisELISAkithasbeentestedinindependentresearchlaboratoriesandfoundtoachievehighestreproducibilitywithintra-andinter-assayCVsaslowas1%and5%,respectively(Polacchinietal.,2015). ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-matureBDNFcaptureantibody,abiotinylatedanti-matureBDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacolouredreactionproductwhichisdirectlyproportionaltotheconcentrationofmatureBDNFpresentinsamplesandproteinstandards.ABDNFpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance. ThisMatureBDNFELISAkitemploysarecombinanthumanmatureBDNFstandardapprovedbytheWorldHealthOrganization(WHO,www.NIBSC.org).TheaminoacidsequenceofmatureBDNFisidenticalforhuman,mouse,ratandanumberofotherspecies.ThiskitthereforeissuitabletomeasurematureBDNFinallthesespeciesandusesthesameantibodiesandantigen. ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures.Forin-vitrodiagnostic(IVD)applicationsintheEuropeanEconomicArea(EEA),werefertotheCEMarkedBDNFRapid^TMELISAkit(BEK-2211-CE).
Relatedproducts	BL-001-1250,SecretoryIgA(sIgA)blocker:SamplediluentadditiveforaccuratequantificationorBDNFinhumanmilk. BEK-2237-1P:HumanproBDNFRapidELISAKit BEK-2240:MatureBDNF/proBDNFComboRapidELISAKit
BatchNo.	Refertotheproductlabel.
Antigen	BDNFbelongstotheneurotrophinfamilyandregulatesthesurvivalanddifferentiationofneuronsduringdevelopment.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"sDisease,andParkinson"sdisease.FUNCTION:BDNFpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.BDNFisalsoamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.PostTranslationModification(PTM):ThepropeptideisN-glycosylatedandglycosulfated.PTM:ConvertedintomatureBDNFbyplasmin(PLG).DISEASE:DefectsinBDNFareacauseofcongenitalcentralhypoventilationsyndrome(CCHS);alsoknownascongenitalfailureofautonomiccontrolorOndinecurse.CCHSisararedisordercharacterizedbyabnormalcontrolofrespirationintheabsenceofneuromuscularorlungdisease,oranidentifiablebrainstemlesion.AdeficiencyinautonomiccontrolofrespirationresultsininadequateornegligIBLeventilatoryandarousalresponsestohypercapniaandhypoxemia.CCHSisfrequentlycomplicatedwithneurocristopathiessuchasHirschsprungdiseasethatoccursinabout16%ofCCHScases.SIMILARITY:BelongstotheNGF-betafamily.
OtherNames	Brain-derivedneurotrophicfactor;BDNF;Abrineurin
Accession	P23560BDNF_HUMAN;
Specificity	Human,mouse,ratandnumerousotherspecies.
Cross-reactivity	Nocross-reactivityisobservedfornervegrowthfactor(NGF),neurotrophin-3(NT-3),NT-4/5,glialcellline-derivedneurotrophicfactor(GDNF)andvascularendothelialgrowthfactor(VEGF165)testedat25ng/mLinassaybuffer.Thereactivityoffull-lengthproBDNF(0.125ng/mL5ng/mL)wasdeterminedinsixindependentassaysusingproBDNFproteinsfromfourdifferentsources(mammalianandbacterial,wild-typeandmutated).Theaveragecross-reactivityofproBDNFwasfoundtobe5.3%±0.5%inweight(w/v)concentration,or12.1%±1.2%inmolarconcentration(mean±SEM).
Storage	Storeat4°C
ExpiryDate	12monthsfrompurchase.
SpecificReferences	ReidlingJCetal.(2017)HumanNeuralStemCellTransplantationRescuesFunctionalDeficitsinR6/2andQ140Huntington"sDiseaseMice.StemCellReports.[Inpress]doi.org/10.1016/j.stemcr.2017.11.005.Application:Mousebrainhomogenates. PerricosA,AshjaeiK,HussleinH,ProestlingK,KuesselL,ObwegeserR,WenzlR,YotovaI(2017)IncreasedserumlevelsofmBDNFinwomenwithminimalandmildendometriosishavenopredictivepowerforthedisease.ExpBiolMed(Maywood).[Epubaheadofprint]doi:10.1177/1535370217742600.Application:Humanserum. InoueT,NinumaS,HayashiM,OkudaA,AsakaT,MaejimaH(2017)Effectsoflong-termexerciseandlow-levelinhibitionofGABAergicsynapsesonmotorcontrolandtheexpressionofBDNFinthemotorrelatedcortex.NeurolRes.11:1-8doi:10.1080/01616412.2017.1382801.Application:Mousebrainhomogenates. PedardM,DemougeotC,PratiC,MarieC(2017)Brain-derivedneurotrophicfactorinadjuvant-inducedarthritisinrats.Relationshipwithinflammationandendothelialdysfunction.ProgNeuropsychopharmacolBiolPsychiatry.pii:S0278-5846(17)30730-3;[Epubaheadofprint]doi:10.1016/j.pnpbp.2017.11.006.Application:Ratserum. WuYJ,LinCC,YehCM,ChienME,TsaoMC,TsengP,HuangCW,HsuKS(2017)Repeatedtranscranialdirectcurrentstimulationimprovescognitivedysfunctionandsynapticplasticitydeficitintheprefrontalcortexofstreptozotocin-induceddiabeticrats.BrainStimul.2017Aug24;[Epubaheadofprint]doi:10.1016/j.brs.2017.08.007.Application:Ratbrainhomogenates. YotovaI,HsuE,DoC,GabaA,SczabolcsM,DekanS,KennerL,WenzlR,TyckoB(2017)EpigeneticAlterationsAffectingTranscriptionFactorsandSignalingPathwaysinStromalCellsofEndometriosis.PLoSOne.2017Jan26;12(1):e0170859.doi:10.1371/journal.pone.0170859.Application:Humanprimarycelllinesupernatants. GuerzoniLPB,NicolasV,AngelovaA(2016)InVitroModulationofTrkBReceptorSignalinguponSequentialDeliveryofCurcumin-DHALoadedCarriersTowardsPromotingNeuronalSurvival.PharmRes.[Epubaheadofprint].doi:10.1007/s11095-016-2080-4.Application:HumancelllinesupernatantsandRIPAextracts. RiffaultB,KourdougliN,DumonC,FerrandN,BuhlerE,SchallerF,ChambonC,RiveraC,GaiarsaJL,PorcherC(2016)Pro-Brain-DerivedNeurotrophicFactor(proBDNF)-Mediatedp75NTRActivationPromotesDepolarizingActionsofGABAandIncreasesSusceptibilitytoEpilepticSeizures.Cereb.Cortex[Epubaheadofprint].doi:10.1093/cercor/bhw385.Application:RatcortexandhippocampusRIPAextracts. KhaingZZ,AgrawalNK,ParkJH,XinS,PlumtonGC,LeeKH,HuangYJ,NiemerskiAL,SchmidtCE,GrauJW(2016)LocalizedandSustainedReleaseofBrain-DerivedNeurotrophicFactorfromInjectableHydrogel/MicroparticleCompositesFostersSpinalLearningafterSpinalCordInjury.JMaterChemB.[Epubaheadofprint].doi:10.1039/C6TB01602B.Application:InvitroreleaseofBDNFfromHAMChydrogels. TurnbullMTandCoulsonEJ(2016)CholinergicBasalForebrainLesionDecreasesNeurotrophinSignalingwithoutAffectingTauHyperphosphorylationinGeneticallySusceptibleMice.JAlzheimersDis.[Epubaheadofprint].doi:10.3233/JAD-160805.Application:Mousehippocampusextracts. BrandliA,JohnstoneDM,StoneJ(2016)RemoteIschemicPreconditioningProtectsRetinalPhotoreceptors:EvidenceFromaRatModelofLight-InducedPhotoreceptorDegeneration.InvestOphthalmolVisSci.57(13):5302-13.doi:10.1167/iovs.16-19361.Application:RatserumandretinalRIPAextracts. ChrysostomouV,GalicS,vanWijngaardenP,TrounceIA,SteinbergGR,CrowstonJG(2016)Exercisereversesage-relatedvulnerabilityoftheretinatoinjurybypreventingcomplement-mediatedsynapseeliminationviaaBDNF-dependentpathway.AgeingCell.2016Sep9.doi:10.1111/acel.12512.[Epubaheadofprint].Application:Mouseserum,mouseretinaandmousemusclehomogenates(Tris/Tritonbuffer). UysM,ShahidM,SallinenJ,DreyerW,CockeranM,HarveyBH(2016)Theα2C-adrenoceptorantagoNIST,ORM-10,921,hasantipsychotic-likeeffectsinsocialisolationrearedratsandbolsterstheeffectsofhaloperidol.ProgNeuropsychopharmacolBiolPsychiatry.2016Jul2.pii:S0278-5846(16)30105-1.doi:10.1016/j.pnpbp.2016.07.002.[Epubaheadofprint].Application:Acid-extractedratbrainhomogenate. PolacchiniA,MetelliG,FrancavillaR,BajG,FloreanM,MascarettiLG,TongiorgiE(2015)AmethodforreproduciblemeasurementsofserumBDNF:comparisonoftheperformanceofsixcommercialassays.SciRep.10;5:17989.doi:10.1038/srep17989.Application:Humanserum. HuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166.Application:Mouseserum. BoskovicZ,AlfonsiF,RumballeBA,FonsekaS,WindelsF,CoulsonEJ(2014)Theroleofp75NTRincholinergicbasalforebrainstructureandfunction.JNeurosci.34(39):13033-8.doi:10.1523/JNEUROSCI.2364-14.2014.Application:Mousebrainhomogenates.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,QCsample,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range	7.8pg/mL-500pg/mL
Sensitivity	Typicallimitofdetection(LOD)forBDNFislessthan2pg/mL,determinedas150%oftheblankvalue.
Kitprotocol	BEK-2211_as_at_June2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf