Biosensis/Mouse Hepatocyte Growth Factor (HGF) ELISA Kit (1 plate)/BEK-2336-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ForquantitativedetectionofmouseHGFincellculturesupernatants,serum,andcitrate,orEDTAtreatedplasmasamplesonlywhenusedasdirected ThemouseHepatocyteGrowthFactor(HGF)KitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseHGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(α)andV496-L728(β))andtestsamplesareaddedtothewells.Abiotinylatedanti-mouseHGFpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofHGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseHGFinsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Range:156pg/mL-10,000pg/mL;Sensitivity<10 pg/mL
BatchNo.	Seeproductlabels
Antigen	Standards(expressionsystemforstandard:NSO;Immunogensequence:Q33-R495(α)andV496-L728(β))
Sequence	Immunogensequence:aminoacids33-495foralphachain,andaminoacids496-728forthebetachainofHGF
OtherNames	Scatterfactor;Hepatopoeitin-A;SF;
Accession	Q08048HGF_MOUSE;
Applications	ELISA
Specificity	NaturalandrecombinantMouseHGF
Cross-reactivity	Thereisnodetectablecross-reactivitywithotherrelevantproteins.
ExpiryDate	Unopenedkitstableat4˚Cfor6months,at-20˚Cfor12months.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	156pg/mL-10,000pg/mL
Sensitivity	< 10 pg/mL
Kitprotocol	Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit.