Biosensis/Mouse Insulin-like growth factor I (IGF-1) ELISA Kit (1 plate)/BEK-2149-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ThemouseInsulin-likegrowthfactorI(IGF-I)KitisasandwichELISA.Thecaptureantibodyispre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownIGF-IconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundIGF-Iantibody.Abiotinylatedanti-mouseIGF-I¶ÿantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofIGF-Ipresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseIGF-Iinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
Antigen	TherecombinantimmunogenandstandardusedinthisELISAwasmanufacturedinanE.coliexpressionsystemsandconsistsofaminoacids49-118ofmouseIGF-1protein.
OtherNames	IGF-I;IGFI;Igf1;IGF-1;IGF1;Insulin-likegrowthfactor-1;
Accession	P05017IGF1_MOUSE;
Specificity	MouseInsulin-likegrowthfactorI
Cross-reactivity	Nodetectablecross-reactivitywithIGF-2.
Storage	Storeat4°C
SpecificReferences	HuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	62.5pg/ml-4,000pg/ml
Sensitivity	< 5 pg/ml
Kitprotocol	Pleasecontactusatbiospeak@biosensis.comforadetailedprotocolforthisELISAkit.