Biosensis/Mouse NT3 ELISA Kit (1 plate)/BEK-2079-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TryournewNT3RapidELISAkitforHuman,RatandMouse(BEK-2221-1P).Samespecificityandsensitivity,butfaster! ThemouseNT3KitisasandwichELISA.ThecaptureantibodyisamonoclonalNT3antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownNT3concentrationareaddedtothesewellsandallowedtocomplexwiththeboundNT3antibody.AbiotinylatedNT3polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofNT3presentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseNT3insamplessuchasseraandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
Antigen	NT3isamemberoftheneurotrophinfamily,thatcontrolssurvivalanddifferentiationofvisceralandproprioceptivesensoryneurons.NT3iscloselyrelatedtobothNGFandBDNF.Itmaybeinvolvedinthemaintenanceoftheadultnervoussystem,andmayaffectdevelopmentofneuronsintheembryowhenitisexpressedinhumanplacenta.NT3-deficientmicegeneratedbygenetargetingdisplayseveremovementdefectsofthelimbs.Thematurepeptideofthisproteinisidenticalinallmammalsexaminedincludinghuman,pig,ratandmouse.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Brainandperipheraltissues.SIMILARITY:BelongstotheNGF-betafamily.
OtherNames	NT-3;NTF3;Neurotrophin-3;HDNF;Nervegrowthfactor2;NGF-2;
Accession	P20181NTF3_MOUSE;
Specificity	Human;Mouse;Rat;
Cross-reactivity	Lessthan2.5%crossreactivitywithNT-4.Nodetectablereactivitywithotherrelevantproteins.
Storage	Storeat4°C
SpecificReferences	DavideD"Amico,ThomasGener,MariaMartínezdeLagrán,MariaVSanchez-Vives,MónicaSantos,MaraDierssen(2016)InfralimbicNeurotrophin-3InfusionRescuesFearExtinctionImpairmentinaMouseModelofPathologicalFear.Neuropsychopharmacology.[Epubaheadofprint]doi:10.1016/j.neulet.2015.12.016. SandraPetzold,BabetteSommer,AndreaKröber,RobertNitsch,HerbertSchwegler,JohannesVogt,ThomasRoskoden(2015)NT-3proteinlevelsareenhancedinthehippocampusofPRG1-deficientmicebutremainunchangedinPRG1/LPA2doublemutants.NeuroscienceLetters.612:145-8doi:10.1016/j.neulet.2015.12.016.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	15.6pg/ml-1,000pg/ml
Sensitivity	< 2 pg/ml
Kitprotocol	BEK-2079-1P_as_at_Oct2016_protocol.pdf