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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
商品详细Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (1 plate)/BEK-2110-1P/1
商品编号: BEK-2110-1P
品牌: Biosensis inc
市场价: ¥8340.00
美元价: 5004.00
产地: 美国(厂家直采)
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产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
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Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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DescriptionThemouseVascularendothelialgrowthfactor(VEGF)KitisasandwichELISA.ThecaptureantibodyisaVEGFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownVEGFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundVEGFantibody.Abiotinylatedanti-mouseVEGFantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofVEGFpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofmouseVEGFinsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.

Vascularendothelialgrowthfactor(VEGF)isadisulfidelinkedhomodimericproteinassociatedwithangiogenesis,vasculogenesisandendothelialcellgrowth.Atleast10isoformsofVEGFareproducedbyalternativesplicingwithvaryinglevelsofexpression.TheisoformsVEGF188,VEGF164andVEGF120arewidelyexpressedwhereastheisoformsVEGF205andVEGF144areuncommon.IsoformsVEGF164andVEGF120arefreelysecretedwhereasisoformVEGF188remainscellassociated.
BatchNo.Seeproductlabels
AntigenThestandardandimmunogenusedintheELISAisrecombinantmouseVEGF-Aaminoacids27-190producedinandpurifiedfromaSF21insectcellexpressionsystem.
OtherNamesVEGF-A;VascularpermeABIlityfactor;VPF;VEGFA;VEGF;VEGF-1(VEGF164);VEGF-2(VEGF120)
AccessionQ00731VEGFA_MOUSE;
SpecificityMouseVEGF.Thiskitrecognizesboththe164and120aminoacidresidueformsofmouseVEGF.
StorageStoreat4°C
SpecificReferencesHuttonCP,DéryN,RosaE,LemonJA,RolloCD,BorehamDR,FahnestockM,deCatanzaroD,WojtowiczJM,BeckerS(2015)Synergisticeffectsofdietandexerciseonhippocampalfunctioninchronicallystressedmice.Neuroscience308:180-93.doi:10.10166.
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range15.6pg/ml-1,000pg/ml
Sensitivity< 2 pg/ml
KitprotocolClick to download the kit protocolBEK-2110-1Plate_as_at_Nov2015protocol.pdf