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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
商品详细Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
Biosensis/Neurotrophin 4/5 (NT4/5) Rapid ELISA Kit: Human, Mouse, Rat and Monkey (1 Plate)/BEK-2218-1P/1 Ea
商品编号: BEK-2218-1P
品牌: Biosensis inc
市场价: ¥7960.00
美元价: 4776.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
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品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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DescriptionTheBiosensisNeurotrophin4/5RapidTMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofNT4/5inlessthan4hoursincellculturesupernatants,humancitrate-plasmaandbrainextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularbloodsamples.AccuratequantificationofNT4/5inhumancitrate-plasmarequiresaheterophilicantibody(HA)blockerwhichcanbepurchasedseparately(BL-003-1000).

ThisELISAkitconsistsofapre-coatedpolyclonalanti-NT4/5captureantibody,abiotinylatedanti-NT4/5detectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproduct,whichisdirectlyproportionaltotheconcentrationofNT4/5presentinsamplesandproteinstandards.

ThisNT4/5ELISAkitemploysarecombinanthumanNT4/5standard.ThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey.

TheantibodiesusedinthiskitbindtoepitopeswithinthematuredomainofNT4/5.Thus,thisELISAkitwillquantifythematureformofNT4/5,andmayalsodetectthefull-lengthpro-formofNT4/5.

ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures.
RelatedproductsBL-003-1000,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationorNT4/5inhumancitrate-plasma.
BatchNo.Refertotheproductlabel.
AntigenFUNCTION:Target-derivedsurvivalfactorforperipheralsensorysympatheticneurons.SUBCELLULARLOCATION:Secretedprotein.TISSUESPECIFICITY:Highestlevelsinprostate,lowerlevelsinthymus,placenta,andskeletalmuscle.Expressedinembryonicandadulttissues.SIMILARITY:BelongstotheNGF-betafamily.
OtherNamesNeurotrophin-5;NT-5;Neutrophicfactor5;Neurotrophin-4;NT-4;Neutrophicfactor4;NTF5;NTF4
AccessionNTF4_HUMAN
SpecificityHuman.
Cross-reactivityThecaptureanddetectionantibodieswillalsodetectNT4/5fromotherspeciesduetoahighdegreeofNT4/5aminoacidsequencehomology.Therefore,thisELISAkitcanbeusedtoquantifyNT4/5inmanyspeciesincludingmouse,ratandmonkey.
StorageStoreat2-8°C.
ExpiryDate12monthsfrompurchase.
SpecificReferencesMaejimaH,KanemuraN,KokubunT,MurataK,TakayanagiK(2017)Exerciseenhancescognitivefunctionandneurotrophinexpressioninthehippocampusaccompaniedbychangesinepigeneticprogramminginsenescence-acceleratedmice.NeurosciLett.doi:10.1016/j.neulet.2017.11.023.[Epubaheadofprint].Application:Mousehippocampushomogenates.

TakahashiK,MaejimaH,IkutaG,ManiH,AsakaT(2016)Exercisecombinedwithlow-levelGABAAreceptorinhibitionup-regulatestheexpressionofneurotrophinsinthemotorcortex.NeurosciLett.doi:10.1016/j.neulet.2016.10.052.[Epubaheadofprint].Application:Mousecortexhomogenates,innativecelllysisbuffer.
GeneralReferencesLodouichi(2000)J.Neuroscie.20(6):2155
Zhangetal.(1999)JNeurosci.Meths.89(1):69
KitcomponentsTheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range15.6-1000pg/mL
SensitivityThisELISAkittypicallydetectsdownto25pg/mLhumanNT4/5(definedas150%ofblankvalue).
KitprotocolClick to download the kit protocolBEK-2218_as_at_March2017_Final.pdf
MSDSClick to download the Material Safety Data SheetMSDS_Rapid_ELISA_as_at_Aug2017.pdf