Biosensis/NGF快速ELISA试剂盒：人（1板）/BEK-2212-1P/1 Ea
商品编号：
BEK-2212-1P
品牌：
Biosensis inc
市场价：
¥7960.00
美元价：
4776.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | The Biosensis NGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of human NGF in less than 3 hours in cell culture supernatants, serum, plasma (citrate) and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum and plasma samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. A human NGF positive control (QC sample) is provided to assure consistent assay performance. This NGF ELISA kit is designed to measure human NGF and thus employs a recombinant human NGF standard approved by the World Health Organization (WHO). Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from numerous other species including mouse and rat! The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, internal validation data demonstrates <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer) with human proNGF protein, suggesting the preferential quantification of mature NGF over full-length human proNGF Note that accurate NGF quantification in human citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-005-500 provided in the kit, and available for purchase separately. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Related products | BL-005-500, Heterophilic antibody blocker: Sample diluent additive for accurate quantification of NGF in human citrate-plasma. |
| Batch No. | Refer to the product label. |
| Antigen | FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family. |
| Other Names | Beta-nerve growth factor; Ngfb |
| Accession | P01138 NGF_HUMAN |
| Specificity | Human |
| Cross-reactivity | The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein and therefore can recognize both the pro- and the mature form of NGF. However, human proNGF protein shows <0.1% cross-reactivity (determined at 25 ng/mL, diluted in assay buffer), suggesting the preferential quantification of mature NGF over full-length human proNGF.
This NGF ELISA does not cross-react with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5, glial cell line-derived neurotrophic factor (GDNF) and vascular endothelial growth factor (VEGF165) tested at 25 ng/mL. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect mature NGF from numerous other species including mouse and rat! |
| Storage | Store at 4°C |
| Expiry Date | 12 months from purchase. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers and detailed protocols. |
| Range | 3.9 - 250 pg/mL |
| Sensitivity | Typical limit of detection (LOD) for human NGF is 2 pg/mL determined as 150% of the blank value. |
| Kit protocol |  BEK-2212_as_at_September2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
TR-200-BG型
PE-1243-50型
抗体试剂盒
PE-1246-5型
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神经营养因子
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电话：PE-1239-5
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2个平板ELISA试剂盒
M-1763-100英尺
TR-200-BGT公司
电话：PE-1239-2
PE-1242-5型
M-1249-100号
神经营养因子受体
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S-1759-100号
PE-1606-100型
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PE-019-100型
PE-1241-5型
PE-003-100
1个平板ELISA试剂盒
BL-003-1000型
PE-1246-2型
感觉神经系统
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TR-100-FJ
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神经内分泌
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PE-1242-2型
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神经递质和酶
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BL-004-500型
PE-019-25
PE-1608-10型
BL-002-1250型
受体体嵌合体
信令与转录
基质和粘附分子
BL-001-1250型
癌症
PE-019-500
干细胞
连接蛋白
TR-300-AG型
细胞凋亡
PE-1252-100型
生长因子
发展
阿尔茨海默氏症/帕金森氏症
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