Biosensis/NGF Rapid ELISA Kit: Mouse (1 Plate)/BEK-2213-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TheBiosensisNGFRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofmouseNGFinlessthan3hoursincellculturesupernatantsandbrainextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication. ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-NGFcaptureantibody,abiotinylatedanti-NGFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofNGFpresentinsamplesandproteinstandards. ThisNGFELISAkitisdesignedtomeasuremouseNGFandthusemploysamouseNGFstandard.ThemouseNGFstandardsuppliedhasbeenpurifiedfrommousesuBMAxillaryglandsaccordingtopublishedprocedures.ThecalibratorstandardreflectsthenativestateofmouseNGFproteinandhasbeenchosentogivemostaccuratequantificationofnaturalNGFproteinlevelsinmousesamples. DuetoahighdegreeofNGFsequencehomology,theantibodiesusedinthiskitwillalsodetectNGFfromotherspeciesincludinghumanandrat!ThisELISAkitpreferentiallydetectsthematureformofNGF.Cross-reactionoffull-lengthmouseproNGFwas< 0.1% when assayed at 25 ng/mL in assay buffer, and not detectable when assayed across the mature NGF calibration range. Thiskithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Refertotheproductlabel.
Antigen	FUNCTION:Nervegrowthfactorisimportantforthedevelopmentandmaintenanceofthesympatheticandsensorynervoussystems.Itstimulatesdivisionanddifferentiationofsympatheticandembryonicsensoryneurons.SUBUNIT:Homodimer,associatedbynon-covalentforces.SUBCELLULARLOCATION:Secretedprotein.SIMILARITY:BelongstotheNGF-betafamily.
OtherNames	Beta-nervegrowthfactor;Ngfb
Accession	NGF_MOUSE
Specificity	Mouse
Cross-reactivity	TheantibodiesusedinthisELISAkitbindepitopeswithinthematuredomainoftheprotein.Nocross-reactivitywasobservedwithbrainderivedneurotrophicfactor(BDNF),neurotrophin-3(NT-3)andNT-4/5testedat25ng/mLinassaybuffer.DuetoahighdegreeofNGFsequencehomology,theantibodiesusedinthiskitwillalsodetectNGFfromotherspeciesincludinghumanandrat! MaturemouseNGF(27kDa)andfull-lengthmouseproNGF(50kDa)wereassayedinparallelatequimolarproteinconcentrationsacrosstheMouseNGFELISAcalibrationrange(3.9-250pg/mL;0.14-9.2pmol/L).ODreadingsformouseproNGFwereindistinguishablefromtheassaysblankODreadings.
Storage	Storeat4°C
ExpiryDate	12monthsfrompurchase.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range	3.9-250pg/mL
Sensitivity	Typicallimitofdetection(LOD)formouseNGFis8pg/mLdeterminedas150%oftheblankvalue.
Kitprotocol	BEK-2213_as_at_Dec2016.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf