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Biosensis/NGF Rapid ELISA Kit: Rat, Guinea Pig (1 Plate)/BEK-2214-1P/1 Ea
Biosensis/NGF Rapid ELISA Kit: Rat, Guinea Pig (1 Plate)/BEK-2214-1P/1 Ea
商品编号：
BEK-2214-1P
品牌：
Biosensis inc
市场价：
¥7960.00
美元价：
4776.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | The Biosensis NGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of rat NGF in less than 3 hours in cell culture supernatants, serum, and brain extracts only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application, in particular serum samples. This ELISA kit consists of a pre-coated mouse monoclonal anti-NGF capture antibody, a biotinylated anti-NGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of NGF present in samples and protein standards. This NGF ELISA kit is designed to measure rat NGF and thus employs a recombinant rat NGF protein. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse! Guinea pig NGF has been quantified in serum using this ELISA kit using rat NGF protein as calibrator, as it shows largest sequence homology based on amino acid sequence among rodent and human NGF proteins. The assay antibodies preferentially detect the mature form of NGF, as shown by data comparing mature mouse NGF and mouse proNGF in the Mouse NGF ELISA kit (BEK-2213). Cross-reaction of full-length proNGF was < 0.1% when assayed at 25 ng/mL in assay buffer, and not detectable when assayed across the mature NGF calibration range. This kit has not been tested for other applications. It has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Batch No. | Refer to the product label. |
| Antigen | FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. SUBUNIT: Homodimer, associated by non-covalent forces. SUBCELLULAR LOCATION: Secreted protein. SIMILARITY: Belongs to the NGF-beta family. |
| Other Names | Beta-nerve growth factor; Ngfb |
| Accession | NGF_RAT |
| Specificity | Rat |
| Cross-reactivity | The antibodies used in this ELISA kit bind epitopes within the mature domain of the protein. No cross-reactivity was observed with brain derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), NT-4/5 and proNGF protein tested at 25 ng/mL in assay buffer. Due to a high degree of NGF sequence homology, the antibodies used in this kit will also detect NGF from other species including human and mouse!
Mature NGF (27 kDa) and full-length proNGF (50 kDa) were assayed in parallel at equimolar protein concentrations across the NGF ELISA calibration range (3.9-250 pg/mL; 0.14-9.2 pmol/L). OD readings for proNGF were indistinguishable from the assays blank OD readings. Data was obtained with the Mouse NGF ELISA kit (BEK-2213) which uses the same assay capture and detection antibodies. |
| Storage | Store at 4°C |
| Expiry Date | 12 months from purchase. |
| Specific References | Cheppudira BP, Trevino AV, Petz LN, Christy RJ, Clifford JL. (2016) "Anti-nerve growth factor antibody attenuates chronic morphine treatment-induced tolerance in the rat." BMC Anesthesiol. 16:73 Application: Acid-extracted rat spinal cord homogenate. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, detection reagents, wash and sample buffers and detailed protocols. |
| Range | 3.9 - 250 pg/mL |
| Sensitivity | Typical limit of detection (LOD) for rat NGF is 8 pg/mL determined as 150% of the blank value. |
| Kit protocol |  BEK-2214_as_at_Jan2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
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