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Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (1 Plate)/BEK-2219-1P/1 Ea
Biosensis/NGFR/p75ECD Rapid ELISA Kit: Human (1 Plate)/BEK-2219-1P/1 Ea
商品编号：
BEK-2219-1P
品牌：
Biosensis inc
市场价：
¥7960.00
美元价：
4776.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | The Biosensis NGFR/p75ECD RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of the extracellular domain (ECD) of human p75 neurotrophin receptor in less than 4 hours in urine, serum, citrate-plasma, CSF and cell culture supernatants only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. Note that capture and detection antibodies have been optimized for increased assay sensitivity, and reagents from previous lots should not be used. This optimized human p75ECD ELISA assay demonstrates an increased sensitivity, lowering the LOD about 8-fold to the previous assay version. It represents the most sensitive NGFR/p75ECD ELISA assay available today, with a detection limit of at least 6 pg/mL, and a quantification range of 7.8 - 500 pg/mL. This ELISA kit consists of a pre-coated mouse monoclonal anti-p75ECD capture antibody, a biotinylated mouse monoclonal anti-p75ECD detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of p75ECD present in samples and protein standards. A human p75ECD positive control (QC sample) is provided to assure consistent assay performance. This NGFR/p75ECD ELISA kit employs an in-house recombinant human p75ECD-Fc chimera as standard that has been validated against an externally available p75ECD-Fc protein. While there is a current lack of a commercially available, true human p75ECD standard, this ELISA kit allows quantification of human p75ECD as p75ECD-Fc human equivalents. Please note that the antibodies used in this ELISA do not cross-react with mouse p75ECD. Note 1: For research use only. Not for diagnostic and clinical purposes. Note 2: This ELISA should not be confused with that reported in the PLoS ONE publication by Shepheard et al (2014, 2017) or any other assay. This kit is unique and has been developed, manufactured and extensively validated in-house and is the most sensitive ELISA available for the detection of p75NTR-ECD in human samples. |
| Related products | BEK-2220-1P, Mouse NGFR/p75ECD RapidTM ELISA BL-002-1250, Heterophilic antibody blocker for ELISA assays utilizing mouse antibodies |
| Batch No. | Refer to the product label. |
| Antigen | The nerve growth factor (NGF) receptor (NGFR), also known as p75 neurotrophin receptor (p75NTR; TNFRS16; CD271) is a common receptor for the neurotrophins NGF, BDNF, NT-3 and NT-4/5. In neurons, p75NTR mediates a variety of physiological functions including survival, apoptosis, neurite outgrowth and synaptic plasticity. A potential pathological role for p75NTR has become evident in recent years. Altered p75NTR expression levels are implicated in degeneration of spinal motor neurons in human and mouse models of amyotrophic lateral sclerosis (ALS). Importantly, the extracellular domain of p75NTR (p75ECD) is shed from the cell membrane and excreted in urine. Recent findings further suggest that p75ECD could be an early biomarker for ALS in humans, as significantly elevated p75ECD levels are found in urine of ALS patients as compared to healthy controls. |
| Other Names | Low-affinity nerve growth factor receptor; NGF receptor; Gp80-LNGFR; p75 ICD; Low affinity neurotrophin receptor p75NTR; Tumor necrosis factor receptor superfamily member 16; CD271; NGFR; TNFRSF16. |
| Accession | TNR16_HUMAN |
| Specificity | Human. |
| Cross-reactivity | This ELISA kit does not cross-react with mouse p75ECD protein. |
| Storage | Store at 2-8°C. |
| Expiry Date | 12 months from purchase. |
| General References | The following references give a general background on the suitability of p75ECD as a biomarker for ALS disease progression. Shepheard et al. (2017) Neurology 2017 Feb 22. doi: 10.1212/WNL.0000000000003741. [Epub ahead of print]. Shepheard et al. (2014) PLoS ONE 9(1): e87398. doi:10.1371/journal.pone.0087398. DiStefano & Johnson (1988) Proc Natl Acad Sci USA 85: 270-274. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers and detailed protocols. |
| Range | 7.8 - 500 pg/mL |
| Sensitivity | Typical limit of detection (LOD) for human p75ECD is less than 6 pg/mL, determined as 150% of the blank value. |
| Kit protocol |  BEK-2219_as_at_Sep2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
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