Biosensis/NGFR/p75ECD Rapid ELISA Kit: Mouse (1 Plate)/BEK-2220-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TheBiosensisNGFR/p75^ECDRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthequantificationofmousep75^ECDinlessthan4hoursncellculturesupernatantsandurineonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication. ThisELISAkitconsistsofapre-coatedmousemonoclonalanti-p75^ECDcaptureantibody,agoatanti-p75^ECDdetectionantibodyandahorserADIshperoxidase(HRP)-conjugatedanti-goatantibody.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofp75^ECDpresentinsamplesandproteinstandards. ThisNGFR/p75^ECDELISAkitemploysarecombinantmousep75^ECD-Fcchimeraproteinasstandard.Whilethereisacurrentlackofatruemousep75^ECDstandard,thisELISAkitallowsquantificationofmousep75^ECDasp75^ECDFcmouseequivalents.PleasenotethattheantibodiesusedinthisELISAcross-reactwithhumanNGFR/p75^ECD. Thiskithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Note:TheBiosensisNGFR/p75^ECDRapid^TMELISAisnotidenticaltotheELISAreportedinShepheardetal.(2014)inPLoSONE.
Relatedproducts	BEK-2219-1P,HumanNGFR/p75^ECDRapid^TMELISA
BatchNo.	Refertotheproductlabel.
Antigen	Thenervegrowthfactor(NGF)receptor(NGFR),alsoknownasp75neurotrophinreceptor(p75NTR;TNFRS16;CD271)isacommonreceptorfortheneurotrophinsNGF,BDNF,NT-3andNT-4/5.Inneurons,p75NTRmediatesavarietyofphysiologicalfunctionsincludingsurvival,apoptosis,neuriteoutgrowthandsynapticplasticity.Apotentialpathologicalroleforp75NTRhasbecomeevidentinrecentyears.Alteredp75NTRexpressionlevelsareimplicatedindegenerationofspinalmotorneuronsinhumanandmousemodelsofamyotrophiclateralsclerosis(ALS).Importantly,theextracellulardomainofp75NTR(p75ECD)isshedfromthecellmembraneandexcretedinurine.Recentfindingsfurthersuggestthatp75ECDcouldbeanearlybioMarkerforALSinhumans,assignificantlyelevatedp75ECDlevelsarefoundinurineofALSpatientsascomparedtohealthycontrols.
OtherNames	Low-affinitynervegrowthfactorreceptor;NGFreceptor;Gp80-LNGFR;p75ICD;Lowaffinityneurotrophinreceptorp75NTR;Tumornecrosisfactorreceptorsuperfamilymember16;CD271;NGFR;TNFRSF16.
Accession	TNR16_MOUSE
Specificity	Mouse.
Cross-reactivity	TheantibodiesusedinthisELISAkitareknowntocross-reactwithhumanp75ECDprotein.
Storage	Storeat2-8°C.
ExpiryDate	12monthsfrompurchase.
SpecificReferences	MaejimaH,KanemuraN,KokubunT,MurataK,TakayanagiK(2017)Exerciseenhancescognitivefunctionandneurotrophinexpressioninthehippocampusaccompaniedbychangesinepigeneticprogramminginsenescence-acceleratedmice.NeurosciLett.doi:10.1016/j.neulet.2017.11.023.[Epubaheadofprint].Application:Mousehippocampushomogenates.
GeneralReferences	DiStefano&Johnson(1988)ProcNatlAcadSciUSA85:270-274. Shepheardetal.(2014)PLoSONE9(1):e87398.doi:10.1371/journal.pone.0087398.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,detectionreagents,washandsamplebuffersanddetailedprotocols.
Range	62.5-4000pg/mL.
Sensitivity	ThisELISAkittypicallydetects20-40pg/mLofmousep75ECD(definedasblankODplus3xthestandarddeviationoftheblankOD,n=10).
Kitprotocol	BEK-2220_as_at_Jan2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf