Biosensis/proBDNF Rapid ELISA Kit: Human (1 Plate)/BEK-2237-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TheBiosensisproBDNFRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofproBDNFinlessthan4hoursincellculturesupernatants,humanserumandEDTA-plasmaonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularhumanbloodsamples. ThisELISAkitconsistsofapre-coatedmonoclonalanti-proBDNFcaptureantibody,abiotinylatedanti-matureBDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3,5,5-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofproBDNFpresentinsamplesandproteinstandards.AproBDNFpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance. ThisproBDNFELISAkitemploysarecombinant,cleavage-resistanthumanproBDNFstandardproducedinmammaliancellsbyBiosensisandvalidatedagainstexternallyavailableproBDNFproteins. NotethataccurateproBDNFquantificationinhumanserumandEDTA-plasmarequirestheadditionofHeterophilicAntibodyBlockerBL-004-500providedinthekit,andavailableforpurchaseseparately. ThisELISAkithasnotbeentestedforotherapplications.Ithasbeenconfiguredforresearchuseonlyandisnottobeusedfordiagnosticorclinicalprocedures.
Relatedproducts	BL-004-500,Heterophilicantibodyblocker:SamplediluentadditiveforaccuratequantificationofproBDNFinhumanserumandEDTA-plasma. BEK-2211-1P:MatureBDNFRapidELISAKit
BatchNo.	Refertotheproductlabel.
Antigen	BDNFbelongstotheneurotrophinfamilyandregulatesthesurvivalanddifferentiationofneuronsduringdevelopment.ThealterationsinBDNFexpressioninducedbyvariouskindsofbraininsultincludingstress,ischemia,seizureactivityandhypoglycemia,maycontributetosomepathologiessuchasdepression,epilepsy,Alzheimer"sDisease,andParkinson"sdisease.FUNCTION:BDNFpromotesthesurvivalofneuronalpopulationsthatarealllocatedeitherinthecentralnervoussystemordirectlyconnectedtoit.BDNFisalsoamajorregulatorofsynaptictransmissionandplasticityatadultsynapsesinmanyregionsoftheCNS.TheversatilityofBDNFisemphasizedbyitscontributiontoarangeofadaptiveneuronalresponsesincludinglong-termpotentiation(LTP),long-termdepression(LTD),certainformsofshort-termsynapticplasticity,aswellashomeostaticregulationofintrinsicneuronalexcitABIlity.SUBUNIT:Monomersandhomodimers.BindstoNTRK2/TRKB.SUBCELLULARLOCATION:Secretedprotein.PostTranslationModification(PTM):ThepropeptideisN-glycosylatedandglycosulfated.PTM:ConvertedintomatureBDNFbyplasmin(PLG).DISEASE:DefectsinBDNFareacauseofcongenitalcentralhypoventilationsyndrome(CCHS);alsoknownascongenitalfailureofautonomiccontrolorOndinecurse.CCHSisararedisordercharacterizedbyabnormalcontrolofrespirationintheabsenceofneuromuscularorlungdisease,oranidentifiablebrainstemlesion.AdeficiencyinautonomiccontrolofrespirationresultsininadequateornegligIBLeventilatoryandarousalresponsestohypercapniaandhypoxemia.CCHSisfrequentlycomplicatedwithneurocristopathiessuchasHirschsprungdiseasethatoccursinabout16%ofCCHScases.SIMILARITY:BelongstotheNGF-betafamily.
OtherNames	Brain-derivedneurotrophicfactor;BDNF;Abrineurin
Accession	P23560BDNF_HUMAN;
Specificity	HumanproBDNF.ThecaptureantibodyusedinthisELISAkitbindstoanepitopewithinthepro-domainofproBDNF.Thus,thisELISAdetectsthefulllengthformofproBDNFanddoesnotquantifymatureBDNF.WhetherthisELISAkitcandetecttruncatedBDNFisunknownatpresent.
Cross-reactivity	MatureBDNFcross-reactivitywasassessedbyspiking28kDamatureBDNFproteinobtainedfromWHO(www.NIBSC.org)intoa1/5dilutedhumanserumsampleat5ng/mL,whichrepresentsaBDNFconcentrationlevelof25ng/mLinundiluted,normalhumanserum.Cross-reactivityofmatureBDNFwas< 0.3% (w/v), or < 0.1% in molar concentration. Theassayantibodiesdonotcross-reactwithnervegrowthfactor(NGF),neurotrophin-3(NT-3)orNT-4/5. Speciescross-reactivityhasnotyetbeendetermined.
Storage	Storeat4°C
ExpiryDate	12monthsfrompurchase.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplate,proteinstandards,QCsample,detectionreagents,heterophilicantibodyblocker,washandsamplebuffersanddetailedprotocols.
Range	15.6-1000pg/mL
Sensitivity	Typicallimitofdetection(LOD)forproBDNFis6pg/mLdeterminedas150%oftheblankvalue.
Kitprotocol	BEK-2237_as_at_December2017.pdf
MSDS	MSDS_Rapid_ELISA_as_at_Aug2017.pdf