TheBiosensisproBDNFRapid^TMenzyme-linkedimmunosorbentassay(ELISA)KitisasandwichELISAthatallowsthespecific,fastandreliablequantificationofproBDNFinlessthan4hoursincellculturesupernatants,celllysates,serum,citrate-plasmaandtissueextractsonlyifusedasdirected.Pleaserefertothekitprotocolforspecificuseinstructionsforeachsubstrateapplication,inparticularhumanbloodsamples.

ThisELISAkitconsistsofapre-coatedpolyclonalanti-proBDNFcaptureantibody,abiotinylatedanti-matureBDNFdetectionantibodyandhorserADIshperoxidase(HRP)-conjugatedstreptavidin.Theadditionofasubstrate(3,3’,5,5’-tetramethylbenzidine,TMB)yieldsacoloredreactionproductwhichisdirectlyproportionaltotheconcentrationofproBDNFpresentinsamplesandproteinstandards.AproBDNFpositivecontrol(QCsample)isprovidedtoassureconsistentassayperformance.

ThisproBDNFELISAkitemploysarecombinant,cleavage-resistanthumanproBDNFstandardproducedbyBiosensisandvalidatedagainstexternallyavailableproBDNFproteins.

Due to a high degree of amino acid sequence homology, mouse and rat proBDNF can be quantified and expressed as human proBDNF equivalents. Internal Biosensis validation suggests that the use of the human standard provided in this kit will provide estimates that are identical, or close, to the actual levels of rat and mouse proBDNF present in rodent samples.

Note that accurate proBDNF quantification in human serum and citrate-plasma requires the addition of Heterophilic Antibody Blocker BL-004-500 provided in the kit, and available for purchase separately.

This ELISA kit has not been tested for other applications. It has been configured for research use only and is not to be used for diagnostic or clinical procedures.