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Biosensis/proNGF Rapid ELISA Kit: Human (1 plate)/BEK-2226-1P/1 Ea
Biosensis/proNGF Rapid ELISA Kit: Human (1 plate)/BEK-2226-1P/1 Ea
商品编号：
BEK-2226-1P
品牌：
Biosensis inc
市场价：
¥7960.00
美元价：
4776.00
产品分类：
夹心法ELISA
公司分类：
Sandwich_method_ELISA
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
| Find a 2 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com | |
| Description | The Biosensis proNGF RapidTM enzyme-linked immunosorbent assay (ELISA) Kit is a sandwich ELISA that allows the quantification of full-length proNGF protein in less than 4 hours in human serum, heparin-plasma, cell supernatants and lysates only if used as directed. Please refer to the kit protocol for specific use instructions for each substrate application. This ELISA kit consists of a pre-coated anti-proNGF capture antibody, a biotinylated anti-proNGF detection antibody and horseradish peroxidase (HRP)-conjugated streptavidin. The addition of a substrate (3,3,5,5-tetramethylbenzidine, TMB) yields a colored reaction product which is directly proportional to the concentration of proNGF present in samples and protein standards. A human proNGF positive control (QC sample) is provided to assure consistent assay performance. This proNGF ELISA kit contains a recombinant human proNGF standard expressed in E.coli. This ELISA kit does not cross-react with the mouse form of proNGF, and due to sequence homology of mouse and rat proNGF is not expected to detect rat proNGF. The antibodies used in this ELISA kit bind epitopes within the pro-domain of the protein and therefore recognize proNGF and the pro-domain peptide, but do not cross-react with mature NGF! Note that accurate proNGF quantification in human serum requires the addition of Heterophilic Antibody Blocker BL-003-1000 provided in the kit, and available for purchase separately. This kit has not been tested for other applications. Sufficient amount of proNGF standard is supplied to allow for spike- and recovery experiments in order to validate this ELISA assay for other sample matrices if required. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. |
| Related products | BL-003-1000, Heterophilic antibody blocker: Sample diluent additive for accurate quantification or proNGF in human serum. |
| Batch No. | Refer to the product label. |
| Antigen | Nerve growth factor (NGF) is synthesized as a precursor (proNGF) which may be released and have physiological functions to cause cell death. It binds neurotrophin receptor p75 and sortilin and may also be important for the development of nervous system. proNGF is synthesized in target tissues and glia, transported retrogradely and may be released. |
| Other Names | pro-brain nerve growth factor; proNGF; NGF |
| Accession | NGF_HUMAN |
| Specificity | Human. Does not react with mouse proNGF, and is not expected to detect rat proNGF. |
| Cross-reactivity | Does not cross-react with recombinant human NGF and proBDNF tested at 25 ng/mL. |
| Storage | Store at 4°C. |
| Expiry Date | 12 months from purchase. |
| Kit components | The ELISA kit box contains 1 x 96 pre-coated strip plate, protein standards, QC sample, detection reagents, heterophilic antibody blocker, wash and sample buffers and detailed protocols. |
| Range | 0.078 - 5 ng/mL |
| Sensitivity | Typical limit of detection (LOD) for proNGF is 30-60 pg/mL determined as blank value plus 3x standard deviation of blank OD (n=10). |
| Kit protocol |  BEK-2226_as_at_Sep2017.pdf |
| MSDS | MSDS_Rapid_ELISA_as_at_Aug2017.pdf |
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司，致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验，并在生命科学研究和诊断市场的生命科学抗体，试剂和试剂盒的商业化方面拥有50多年的经验。
品牌分类
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2个平板ELISA试剂盒
M-1763-100英尺
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M-1249-100号
神经营养因子受体
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1个平板ELISA试剂盒
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感觉神经系统
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BL-001-1250型
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PE-019-500
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TR-300-AG型
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发展
阿尔茨海默氏症/帕金森氏症
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