Biosensis/Rat Glial cell line-derived neurotrophic factor/GDNF/ATF ELISA(1 plate)/BEK-2020-1P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	TryournewGDNFRapidELISAkit(BEK-2230-1P).Samespecificity,moresensitivityandfaster! TheratGDNFKitisasandwichELISA.Thecaptureantibodyisamonoclonalanti-ratGDNFantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.RattestsamplesandstandardsofknownGDNFconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundGDNFantibody.Abiotinylatedant-ratGDNFpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofGDNFpresentinthesamples.ThepurposeofthiskitisforquantitativedetectionofratGDNFincellculturesupernatants,celllysates,tissuehomogenates,serum,andheparinorEDTAtreatedplasmasamplesonlywhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. GDNFisaglycosylated,disulfide-bondedhomodimermolecule.ItwasfirstdiscoveredasapotentsurvivalfactorformidbraindopaminergicneuronsandwasthenshowntorescuetheseneuronsinanimalmodelsofParkinson"sdisease.GDNFisabout100timesmoreefficientsurvivalfactorforspinalmotorneuronsthantheneurotrophins.FUNCTION:Neurotrophicfactorthatenhancessurvivalandmorphologicaldifferentiationofdopaminergicneuronsandincreasestheirhigh-affinitydopamineuptake.SUBUNIT:Homodimer;disulfide-linked.SUBCELLULARLOCATION:Secretedprotein.ALTERNATIVEPRODUCTS:2namedisoformsproducedbyalternativesplicing.SIMILARITY:BelongstotheTGF-betafamily.GDNFsubfamily.
BatchNo.	Seeproductlabels
Antigen	Thestandardandimmunogenusedinthiskitisrecombinantmature(aa78-211)ratGDNFproducedinandpurifiedfromaninsectcellexpressionsystem.ThisassaywillnotdistinguishbetweenunprocessedproGDNFandmatureGDNFastheantibodieswilldetectbothformswhenpresent.
Sequence	>sp\|Q07731\|78-211SPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI
OtherNames	Glialcellline-derivedneurotrophicfactor;Astrocyte-derivedtrophicfactor1;ATF-1
Accession	Q07731GDNF_RAT;
Purity	ThestandardinthiskitwasexpressedinandpurifiedfromanSf21baculovirussystem.
Applications	ELISA
Specificity	RatGDNF&proGDNF
Cross-reactivity	CrossreactivitytohumanandmouseGDNFislikelybecauseofsequencehomology
Storage	Storeat4øC
SpecificReferences	SimõesLetal.(2017)"PreventionofmemoryimpairmentandneutrophicfactorsincreasedbylithiuminWistarratssubmittedtopneumococcalmeningitismodel."MediatorsInflamm.Sep;[Epubaheadofprint] ValvassoriSSetal.(2017)"Thedifferenteffectsoflithiumandtamoxifenonmemoryformationandthelevelsofneurotrophicfactorsinthebrainofmaleandfemalerats."BrainResBull.Sep;134:228-35 VarelaRBetal.(2015)"SodiumbutyrateandmoodstABIlizersblockouabain-inducedhyperlocomotionandincreaseBDNF,NGFandGDNFlevelsinbrainofWistarrats."JPsychiatrRes.Feb;61:114-121. BarichelloTetal.(2015)"SodiumButyratePreventsMemoryImpairmentbyRe-establishingBDNFandGDNFExpressioninExperimentalPneumococcalMeningitis."MolNeurobiol.Aug;52(1):734-40. LeffaDDetal.(2015)"Effectsofpalatablecafeteriadietoncognitiveandnoncognitivebehaviorsandbrainneurotrophins"levelsinmice."MetabBrainDis.Aug;30(4):1073-82.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	31.2pg/ml-2,000pg/ml
Sensitivity	< 4 pg/ml
Kitprotocol	BEK-2020-1P_as_at_Feb2017_protocol.pdf