Biosensis/Human Endothelin-1/ET-1/EDN1 ELISA Kit (2 plates)/BEK-2334-2P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ForquantitativedetectionofhumanEndothelinincellculturesupernatants,serum,andheparin,orEDTAtreatedplasmasamplesonlywhenusedasdirected ThehumanEndothelinKitisasandwichELISA.ThecaptureantibodyisamonoclonalmouseEndothelinantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.Standards(expressionsystemforstandard:humanpeptide;Immunogensequence:C53-W73)andtestsamplesareaddedtothewells.Abiotinylateddetectionpolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofEndothelinpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanEndothelininsamplessuchassera,plasma,andcellculturesupernates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures. Range:3.9pg/mL-250pg/mL;Sensitivity<0.5 pg/mL
BatchNo.	Seeproductlabels
Antigen	Standards(expressionsystemforstandard:humanpeptide;Immunogensequence:C53-W73).
Accession	P05305EDN1_HUMAN
Applications	ELISA
Specificity	NaturalandrecombinantHumanEndothelin
Cross-reactivity	Thereisnodetectablecross-reactivitywithotherrelevantproteins.
ExpiryDate	Storeat4˚Cfor6months,at-20˚Cfor12months.
Kitcomponents	TheELISAkitboxcontains1x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	3.9pg/mL-250pg/mL
Sensitivity	< 0.5 pg/mL
Kitprotocol	BEK-2334-2P_as_at_Nov2015_protocol.pdf