生物传感器/人类肿瘤坏死因子α/Cachectin/TNFSF2 ELISA试剂盒（2片）/BEK-2100-2P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	Tumornecrosisfactoralpha(TNF-alpha)isacytokinethatbelongstothetumornecrosisfactor(TNF)superfamily.TNF-alphaismainlysecretedbymacrophages.TNF-alphaisinvolvedintheregulationofmanyprocessessuchascellproliferation,differentiationandapoptosisandhasbeenimplicatedinavarietyofdiseasesincludingcancer.TNF-alphaisexpressedasasingle-passtypeIImembraneprotein.Asoluble,secretedformisderivedfromthemembraneformbyproteolyticprocessing. ThehumanTNF-alphaKitisasandwichELISA.ThecaptureantibodyisamonoclonalhumanTNF-alphaantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownTNF-alphaconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundTNF-alphaantibody.AbiotinylatedhumanTNF-alphapolyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTNF-alphapresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedeterminationofhumanTNF-alphainsamplessuchassera,plasma,tissuelysatesandcellculturesupernates.TForquantitativedetectionofhumanTNFαincellculturesupernatants,serum,andheparin,EDTAorcitratetreatedplasmasamplesonlywhenusedasdirected.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.	Seeproductlabels
Antigen	ThestandardusedinthishumanTNFalphakitwasalsousedastheantigenfortheantibodies.Itisarecombinanthumanproteinfragmentexpressedine.coliandpurified.TheexactsequencemapstothesolubleformofTNFalpha,aminoacids77-233.
Sequence	>sp\|P01375\|77-233VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
OtherNames	TNF-alpha;Tumornecrosisfactorligandsuperfamilymember2;TNF-a;Cachectin;TNF;TNFA;TNFSF2;
Accession	P01375TNFA_HUMAN;
Specificity	HumanTNF-alpha
Storage	Storeat4°C
Kitcomponents	TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	15.6pg/ml-1,000pg/mlforbodyfluids,tissuelysatesorcellculturesupernates.7.8pg/ml-500pg/mlforhumansera.
Sensitivity	< 1 pg/ml
Kitprotocol	BEK-2100-2P_as_at_Nov2017_protocol.pdf