Biosensis/Human Transforming growth factor beta-1 ELISA Kit (2 plates)/BEK-2093-2P/1 Ea-免疫在线蚂蚁淘旗下平台

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Description	ThehumanTransforminggrowthfactorbeta-1(TGFb1)KitisasandwichELISA.ThecaptureantibodyisamonoclonalTGFb1antibodypre-coatedontothe96-wellstripplatesprovidedinthekit.HumantestsamplesandstandardsofknownTGFb1concentrationareaddedtothesewellsandallowedtocomplexwiththeboundTGFb1antibody.AbiotinylatedTGFb1polyclonalantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofTGFb1presentinthesamples.Thereiscross-reactivateswithTGFbeta2,TGFbeta3,TGFbeta5<1%. The purpose of this kit is the in-vitro quantitative determination of human TGFb1 in cell culture supernatants, serum, plasma (EDTA treated only) and urine samples. Pretreatment of samples with acid precipitation and neutralization step is required for optimal activity. Note: TGFbeta 1 is naturally very liable once released from its natural binding complex. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
BatchNo.	Seeproductlabels
OtherNames	TGFB1;TGFB;TGF-beta-1;
Accession	P01137TGFB1_HUMAN;
Specificity	TGFBeta1.ThekitwillreactwithHuman,MouseandRat.
Cross-reactivity	CrossreactivitywithTGFBeta2,TGFBeta3,andTGFBeta5islessthan1%.
Storage	Storeat4°C
Kitcomponents	TheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range	15.6pg/ml-1,000pg/ml
Sensitivity	< 1 pg/ml
Kitprotocol	BEK-2093-2P_as_at_Jun2017_protocol.pdf