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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Biosensis/Mouse P-selectin (sP-selectin)/ GMP-140/LECAM3/PADGEM/CD62P ELISA Kit (2 plates)/BEK-2091-2P/1 Ea
商品详细Biosensis/Mouse P-selectin (sP-selectin)/ GMP-140/LECAM3/PADGEM/CD62P ELISA Kit (2 plates)/BEK-2091-2P/1 Ea
Biosensis/Mouse P-selectin (sP-selectin)/ GMP-140/LECAM3/PADGEM/CD62P ELISA Kit (2 plates)/BEK-2091-2P/1 Ea
Biosensis/Mouse P-selectin (sP-selectin)/ GMP-140/LECAM3/PADGEM/CD62P ELISA Kit (2 plates)/BEK-2091-2P/1 Ea
商品编号: BEK-2091-2P
品牌: Biosensis inc
市场价: ¥15940.00
美元价: 9564.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
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品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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DescriptionThemouseP-SelectinKitisasandwichELISA.Themousecaptureantibodyisananti-mouseP-Selectinantibodypre-coatedontothe96-wellstripplatesprovidedinthekit.MousetestsamplesandstandardsofknownP-SelectinconcentrationareaddedtothesewellsandallowedtocomplexwiththeboundP-Selectinantibody.Abiotinylatedanti-mouseP-Selectinantibodyisthenadded.Thisdetectionantibodybindstotheantigenthuscompletingthesandwich.Afterwashing,anenzymeAvidin-Biotin-Peroxidasecomplex(ABC)isaddedwhichbindstothesecondantibody.TheperoxidasesubstrateTMBisaddedtoinduceacolouredreactionproduct.TheintensityofthiscolouredproductisdirectlyproportionaltotheconcentrationofP-Selectinpresentinthesamples.Thepurposeofthiskitisthein-vitroquantitativedetectionofmouseP-Selectinincellculturesupernatants,serumandcitratetreatedplasmasamplesonlywhenusedasdirected.Thisassayhasnotbeentestedontissuelysates.Thiskithasbeenconfiguredforresearchuseonlyandisnottobeusedindiagnosticorclinicalprocedures.
BatchNo.Seeproductlabels
AntigenStandardisrecombinantmouseP-selectin,aminoacids42-709expressedinandpurifiedfromamammaliancellexpressionsystem.Aminoacids42-709representtheECDdomainofmouseP-selectin,sothisELISAwilldetectprimarilysolubleP-selectininitstargetedsubstratesbutfullylengthP-selectinifavailablecouldcompeteaswell.
OtherNamesCD62antigen-likefamilymemberP;Granulemembraneprotein140;GMP-140;Leukocyte-endothelialcelladhesionmolecule3;LECAM3;Plateletactivationdependentgranule-externalmembraneprotein;PADGEM;CD62P;SELP;GMRP;GRMP;
AccessionQ01102LYAM3_MOUSE;
ApplicationsELISA
SpecificityMouseP-Selectin,solubleP-Selectin,s(P-Selectin)
StorageStoreat4°C
KitcomponentsTheELISAkitboxcontains2x96pre-coatedstripplates,proteinstandards,detectionreagents,substratebufferandpreciseinstructions.
Range312pg/mL-20000pg/mL
Sensitivity< 5 pg/ml
KitprotocolClick to download the kit protocolBEK-2091-2P_as_at_Feb2017_protocol.pdf