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当前位置: 首页 > 产品中心 > Sandwich_method_ELISA > Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (2 plates)/BEK-2110-2P/
商品详细Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (2 plates)/BEK-2110-2P/
Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (2 plates)/BEK-2110-2P/
Biosensis/Mouse Vascular endothelial growth factor A/VEGF-A/VEGF-164/VEGF-1/VEGF-120/VEGF-2 ELISA Kit (2 plates)/BEK-2110-2P/
商品编号: BEK-2110-2P
品牌: Biosensis inc
市场价: ¥15940.00
美元价: 9564.00
产地: 美国(厂家直采)
公司:
产品分类: 夹心法ELISA
公司分类: Sandwich_method_ELISA
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
  Find a 1 plate size of this product here. Enquire about bulk discounts for ten or more plates, at sales@biosensis.com
Description The mouse Vascular endothelial growth factor (VEGF) Kit is a sandwich ELISA. The capture antibody is a VEGF antibody pre-coated onto the 96-well strip plates provided in the kit. Mouse test samples and standards of known VEGF concentration are added to these wells and allowed to complex with the bound VEGF antibody. A biotinylated anti-mouse VEGF antibody is then added. This detection antibody binds to the antigen thus completing the sandwich. After washing, an enzyme Avidin-Biotin-Peroxidase complex (ABC) is added which binds to the second antibody. The peroxidase substrate TMB is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of VEGF present in the samples. The purpose of this kit is the in-vitro quantitative determination of mouse VEGF in samples such as sera, plasma, tissue lysates and cell culture supernates. This kit has been configured for research use only and is not to be used in diagnostic or clinical procedures.
Vascular endothelial growth factor (VEGF) is a disulfide linked homodimeric protein associated with angiogenesis, vasculogenesis and endothelial cell growth. At least 10 isoforms of VEGF are produced by alternative splicing with varying levels of expression. The isoforms VEGF188, VEGF164 and VEGF120 are widely expressed whereas the isoforms VEGF205 and VEGF144 are uncommon. Isoforms VEGF165 and VEGF120 are freely secreted whereas isoform VEGF188 remains cell associated.
Batch No. See product labels
Antigen The standard and immunogen used in this kit is recombinant mouse VEGF-A amino acids 27-190 produced in and purified from a SF21 insect cell expression system.
Other Names VEGF-A; Vascular permeability factor; VPF; VEGFA; VEGF; VEGF-1' VEGF164; VEGF-2; VEGF120
Accession Q00731 VEGFA_MOUSE;
Specificity Mouse VEGF. This kit recognizes both the 164 and 120 amino acid residue forms of mouse VEGF.
Storage Store at 4°C
Specific References Hutton CP, Déry N, Rosa E, Lemon JA, Rollo CD, Boreham DR, Fahnestock M, deCatanzaro D, Wojtowicz JM, Becker S (2015) Synergistic effects of diet and exercise on hippocampal function in chronically stressed mice. Neuroscience 308:180-93. doi: 10.10166.
Kit components The ELISA kit box contains 2 x 96 pre-coated strip plates, protein standards, detection reagents, substrate buffer and precise instructions.
Range 15.6 pg/ml - 1,000 pg/ml
Sensitivity < 2 pg/ml
Kit protocol Click to download the kit protocolBEK-2110-2P_as_at_Nov2015_protocol.pdf
品牌介绍
Biosensis是一家由生命科学家和商业团队组成的公司,致力于为全球学术界和工业界的研究人员提供支持。该团队在基础和应用生命科学研究方面拥有超过100年的经验,并在生命科学研究和诊断市场的生命科学抗体,试剂和试剂盒的商业化方面拥有50多年的经验。
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